diff --git a/CHANGELOG.md b/CHANGELOG.md
index 692a1914c..1c4336d17 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -2,7 +2,8 @@
 
 ## Master (Unreleased)
 
-- Support correcting `assert_nil` and `refute_nil` to `RSpec/Rails/MinitestAssertions`. ([@G-Rath])
+- Support correcting `assert_nil` and `refute_nil` in `RSpec/Rails/MinitestAssertions`. ([@G-Rath])
+- Support correcting `assert_not_equal` and `assert_not_equal` in `RSpec/Rails/MinitestAssertions`. ([@G-Rath])
 - Fix a false positive for `RSpec/ExpectActual` when used with rspec-rails routing matchers. ([@naveg])
 - Add new `RSpec/RepeatedSubjectCall` cop. ([@drcapulet])
 
diff --git a/lib/rubocop/cop/rspec/rails/minitest_assertions.rb b/lib/rubocop/cop/rspec/rails/minitest_assertions.rb
index f12448691..15e53c3d3 100644
--- a/lib/rubocop/cop/rspec/rails/minitest_assertions.rb
+++ b/lib/rubocop/cop/rspec/rails/minitest_assertions.rb
@@ -29,19 +29,21 @@ class MinitestAssertions < Base
           MSG = 'Use `%<prefer>s`.'
           RESTRICT_ON_SEND = %i[
             assert_equal
+            assert_not_equal
             refute_equal
             assert_nil
+            assert_not_nil
             refute_nil
           ].freeze
 
           # @!method minitest_equal_assertion(node)
           def_node_matcher :minitest_equal_assertion, <<~PATTERN
-            (send nil? {:assert_equal :refute_equal} $_ $_ $_?)
+            (send nil? {:assert_equal :assert_not_equal :refute_equal} $_ $_ $_?)
           PATTERN
 
           # @!method minitest_nil_assertion(node)
           def_node_matcher :minitest_nil_assertion, <<~PATTERN
-            (send nil? {:assert_nil :refute_nil} $_ $_?)
+            (send nil? {:assert_nil :assert_not_nil :refute_nil} $_ $_?)
           PATTERN
 
           def on_send(node)
diff --git a/spec/rubocop/cop/rspec/rails/minitest_assertions_spec.rb b/spec/rubocop/cop/rspec/rails/minitest_assertions_spec.rb
index 7537060be..d1e817a61 100644
--- a/spec/rubocop/cop/rspec/rails/minitest_assertions_spec.rb
+++ b/spec/rubocop/cop/rspec/rails/minitest_assertions_spec.rb
@@ -49,6 +49,17 @@
       RUBY
     end
 
+    it 'registers an offense when using `assert_not_equal`' do
+      expect_offense(<<~RUBY)
+        assert_not_equal a, b
+        ^^^^^^^^^^^^^^^^^^^^^ Use `expect(b).not_to eq(a)`.
+      RUBY
+
+      expect_correction(<<~RUBY)
+        expect(b).not_to eq(a)
+      RUBY
+    end
+
     it 'registers an offense when using `refute_equal`' do
       expect_offense(<<~RUBY)
         refute_equal a, b
@@ -120,6 +131,17 @@
       RUBY
     end
 
+    it 'registers an offense when using `assert_not_nil`' do
+      expect_offense(<<~RUBY)
+        assert_not_nil a
+        ^^^^^^^^^^^^^^^^ Use `expect(a).not_to eq(nil)`.
+      RUBY
+
+      expect_correction(<<~RUBY)
+        expect(a).not_to eq(nil)
+      RUBY
+    end
+
     it 'registers an offense when using `refute_nil`' do
       expect_offense(<<~RUBY)
         refute_nil a