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ID SPBC1215 standard; DNA; FUN; 6490 BP.
XX
AC AL096846;
XX
DE S.pombe chromosome II cosmid c1215.
XX
KW SURF-family protein; COX complex biogenesis; DEC1 homologue;
KW mitochondrial inheritance; actin cytoskeleton organisation.
XX
OS Schizosaccharomyces pombe (yeast)
OC Eukaryota; Plantae; Thallobionta; Eumycota; Hemiascomycetes;
OC Endomycetales; Saccharomycetaceae.
XX
RN [1]
RP 1-6490
RA Lyne M.H., Rajandream M.A., Barrell B.G., Seeger K., Quail M., Harris D.;
RT ;
RL Submitted (16-JUL-1999) to the EMBL/GenBank/DDBJ databases.
RL European Schizosaccharomyces genome sequencing project,
RL Sanger Centre, The Wellcome Trust Genome Campus, Hinxton,
RL Cambridge CB10 1SA, E-mail: barrell@sanger.ac.uk and
XX
CC Notes:
CC
CC Details of yeast sequencing at the Sanger Centre are available on
CC the World Wide Web.
CC (URL, http://www.sanger.ac.uk/Projects/S_pombe/)
CC During 1995 to 1996 about 66% of S. pombe chromosome 1 was sequenced
CC by the Sanger Centre. The sequencing of the S. pombe genome is now
CC being continued with funding from The European Commission.
CC Fourteen European sequencing laboratories, including the Sanger Centre,
CC are participating in the project.
CC
CC Protein coding regions (CDS) have been predicted with the help
CC of computer analysis using the Genefinder program in PomBase
CC (an ACEDB database) with additional predictions for the
CC branch-acceptor sites supplied by the program Sp3splice.
CC CAUTION: It is possible that for any individual CDS we may have
CC underestimated or overestimated the number of introns/exons or
CC we may not have chosen the correct splice donor/acceptor sites.
CC
CC CDS are numbered using the following system eg SPBC25H2.01c.
CC SP (S. pombe), B (chromosome 2), c25H2 (cosmid name),
CC .01 (first CDS), c (complementary strand).
CC
CC The more significant matches with motifs in the PROSITE
CC database are also included but some of these may be fortuitous.
CC
CC The length in codons is given for each CDS.
CC
CC IMPORTANT: This sequence MAY NOT be the entire insert of
CC the sequenced clone. It may be shorter because we only
CC sequence overlapping sections once, or longer, because we
CC arrange for a small overlap between neighbouring submissions.
CC
CC Cosmid c1215 is overlapped at the 5' end by cosmid c1750,
CC EMBL entry SPAB4534, accession number AB004534, and at the 3'
CC end by cosmid c83, EMBL entry SPBC83, accession number AL035536.
XX
XX
FH Key Location/Qualifiers
FH
FT source 1..6490
FT /organism="Schizosaccharomyces pombe"
FT /strain="972h-"
FT /chromosome="II"
FT /clone="cosmid c1215"
FT /map="IIL"
FT misc_feature complement(1..82)
FT /note="nominal overlap with cosmid SPAB4534 (c1750) S.
FT pombe chromosome 2"
FT tRNA 36..107
FT /note="tRNA Thr anticodon TGT, Cove score 78.51"
FT rRNA 415..581
FT /note="SPRG5SD K00771 Yeast (s.pombe) 5s rrna gene and
FT flanks"
FT CDS join(1501..1760,1844..2456)
FT /fasta_file="fasta/gf.tab.seq.00001.out"
FT /note="SPBC1215.01, len:290, SIMILARITY:Saccharomyces
FT cerevisiae, YG2X_YEAST, hypothetical 45.1 kd protein in
FT clb6-spt6 intergenic region, (389 aa), fasta scores: opt:
FT 385, E():2.8e-19, (29.0% identity in 314 aa)"
FT /gene="SPBC1215.01"
FT /product="putative SURF-family protein"
FT /colour=7
FT /label=SPBC1215.01
FT misc_feature 1761..1766
FT /note="gtacgt, splice donor sequence"
FT /colour=6
FT misc_feature 1827..1843
FT /note="ctaacataatcacttag, splice branch and acceptor"
FT /colour=6
FT CDS complement(join(2495..2602,2663..2881,2953..4071,4111..4372,4413..4849,4889..5007,5251..5389,5434..5466))
FT /fasta_file="fasta/gf.tab.seq.00002.out"
FT /note="SPBC1215.02c, len:811, SIMILARITY:Saccharomyces
FT cerevisiae, DEC1_YEAST, dec1 protein, (796 aa), fasta
FT scores: opt: 184, E():0.00014, (23.4% identity in 577 aa)"
FT /gene="SPBC1215.02c"
FT /product="similar to yeast DEC1 mitochondrial inheritance
FT and actin cytoskeleton organisation protein"
FT /colour=7
FT /label=SPBC1215.02c
FT misc_feature complement(2603..2612)
FT /note="ctaataatag, splice branch and acceptor"
FT /colour=6
FT misc_feature complement(2657..2662)
FT /note="gtacgt, splice donor sequence"
FT /colour=6
FT misc_feature complement(2882..2896)
FT /note="ctaaccgaattatag, splice branch and acceptor"
FT /colour=6
FT misc_feature complement(2947..2952)
FT /note="gtaagt, splice donor sequence"
FT /colour=6
FT misc_feature complement(4072..4085)
FT /note="ttaactcgtcgtag, splice branch and acceptor"
FT /colour=6
FT misc_feature complement(4105..4110)
FT /note="gtaagt, splice donor sequence"
FT /colour=6
FT misc_feature complement(4373..4387)
FT /note="ctaacttctccatag, splice branch and acceptor"
FT /colour=6
FT misc_feature complement(4407..4412)
FT /note="gtaagt, splice donor sequence"
FT /colour=6
FT misc_feature complement(4850..4861)
FT /note="ctaactcctcag, splice branch and acceptor"
FT /colour=6
FT misc_feature complement(4883..4888)
FT /note="gtaaga, splice donor sequence"
FT /colour=6
FT misc_feature complement(5008..5021)
FT /note="ttaactatttgaag, splice branch and acceptor"
FT /colour=6
FT misc_feature complement(5245..5250)
FT /note="gtaagt, splice donor sequence"
FT /colour=6
FT misc_feature complement(5390..5405)
FT /note="ttaacatcctttttag, splice branch and acceptor"
FT /colour=6
FT misc_feature complement(5428..5433)
FT /note="gtaagt, splice donor sequence"
FT /colour=6
FT misc_feature 6387..6490
FT /note="nominal overlap with cosmid SPBC83 S. pombe
FT chromosome 2"
SQ Sequence 6490 BP; 2097 A; 1138 C; 1138 G; 2117 T; 0 other;
tatatataat ttaataaata cattccgacg atactgcctc tatggcttag tggtacagca 60
tcgcacttgt aatgcgaaga tccttggttc gattccgagt ggaggcatat acattatatt 120
atattctttt tcatgcggaa aaaagatttc aaatttttgg gtatgatatt aatatgactg 180
taacgttaat agcaaagtga gtgttaataa tgataaaata gcagcaaaat ctcttttccg 240
agtaagacgt tttccagtct aaatttggag tctgcagttg tttcgcaatt cttaatgtat 300
ggttatacta aatacaaact ttaaagctct gatttatgtt tgcaataaac taaaataaaa 360
gcacaaaaac ctttacccat taatttcaaa caacttataa actaccggta aacttttttt 420
ctaaccttta taatttataa actagaatgt ttaatgtcta cggccatacc taggcgaaaa 480
caccagttcc cgtccgatca ctgcagttaa gcgtctgagg gcctcgttag tactatggtt 540
ggagacaaca tgggaatccg gggtgctgta ggctattttt ttatatccgt ctttcttact 600
acttgcctaa caagtcatga tgtactctca aaatatgttt gcatgccttg taatattggt 660
tatggatagc tccttctgga cttgatcttt tgtagccaag aacaatgggt atagactctg 720
accttgtgat gttgtagcca cagattataa taggtatttt caagtacagt aacaaaaatc 780
ttctagtttt tttttagaaa ggatacacca agtataagca aattcaggaa ttgttgatta 840
aactgtcaac ttcggtaaaa ctttgggcat aagtagtgtg ggagcaagtt taactaaaat 900
tctattcaga tgtcgaatcc aaaccgctaa ttttgctcaa ctagcttttc ataaaaacca 960
attcatagtt tcatactaat aaagacgatt gtttacttta aaacatacgt cgtaagaaca 1020
tatattgctt tatcgaaaga taacaaatgt taagctatta tattatttaa ctatagcgca 1080
gatttcgctt cctttactta aaaaagacat gtgacttgta gaagcttgga gtgaatacgc 1140
aaaggtacct acttagacat tcgcgtctct cttagctgtc aacatcaaca aactggcccc 1200
gtattgaaca gtatcttact tgtcgaagga tttgactaag aaaattttat ttctttatag 1260
caatattccg ttttcgctta gaagattcta gtcaattgcc ctattctact tacgctttac 1320
agtagtatca gaagacctga gtgggatttt gctgctagta gaggccattc aagttaactc 1380
cgttttgcaa cattttaaaa gtttttgaat tgaatataaa tatcaattgt ttgattcctt 1440
ttaggattta atcttttctt tttatttttg tttcgattga atcttggatt cctgtctatc 1500
atgttttggt ggaaaagtgc tactaaattc acattctcaa agcgtggacc gtgtgtcttt 1560
cgctatttga gtactcttga aggaacaact gtgaggccta aaaaaaataa atttttagtt 1620
ggattgcttt ctgccgttcc aattgtcacg tttgctttag gaacttggca ggtaaagcga 1680
cgagaatgga aaatgggtat catcaataca ctcacggaaa ggcttcaaca gcccgcaatt 1740
ttattaccga aaactgttac gtacgttaag ttaacatata cacaaattgc acgttttgca 1800
attgaactgt cgttttttac attaagctaa cataatcact tagagagcaa gatacaaaaa 1860
aacttgagtg gactagggtt ttgcttcgtg gtgtgttttg tcacgaccaa gaaatgttgg 1920
tcggtccaag aacgaaggaa ggccaacctg gctatcacgt agtaacccca tttattttag 1980
acgatgggcg tcgaatttta gtcaacagag gatggattgc tcgatcattt gctgaacagt 2040
cttctcgaga tcctagttct ttacctaaag gtccagtggt cattgaaggt cttttgagac 2100
aacatactga taagccaaga tttatgatga agaatgagcc tgaaaaaaat tctttttact 2160
tcttaaatgt tcgtgagttt gcacaattga aaggaactct ccccattttg ataacagaac 2220
tacaaccatc gcttacaccg ttgcaagaag ccgatcatgt taagagaggc ttgcctcttg 2280
gtcatcctct aaaagttgaa attttcaaca gtcatacaga atatattatc acttggtatt 2340
ctctaagtgt ggtatcagct ataatgcttt acgtctattt taagagaggt tcaggcacat 2400
cttctctgaa ttctgcatac gaaagaagca agattctaaa caacaaacga ttataaaaaa 2460
ttttcatatt tataagtttc taaatattat ctacctaaaa ttttacaaat tttggaagct 2520
tgcttactgc gtccgtcgtt tgaatgtatg aatcgatcat tccttcacca acttgttttg 2580
caaacttcgg atcgtaagga acctattatt aggaagttag ttctcatgcc tataatttta 2640
atgctctata atcatcacgt acctgagttt cagataaatt gctcagccaa gagtttgaca 2700
acaattctga tacatatttt ctggcggcct tctttttatg cttcgtaatt ccagagatac 2760
ttcctaattt gttactgctg atgtttttaa gaagttggta ttgtctggta aattcttttt 2820
tcttggtagc tgacacgtga tataggaagc tgtttagaca agtgataagg tcatttatga 2880
tctataattc ggttagttta gaaattttta tatcattttt tttaaaaaaa aaaaccagtt 2940
tggtaaactt acttcagtgt actttgtcaa ttgacttaaa ggcgtggagt tctcataatc 3000
gaatgactca attaaatttt caatcgtttc gaacgaggaa ttttcgtagt ctccgttctt 3060
gacctttacc gaaagtaatc ccagttggat caataatttc atatgaacaa tttcttccga 3120
agttaattgc ttcgataaat cattgttttc gcacaaaact tccatttcct tagcacttaa 3180
aacggccttt tcaaaatcgc cgtttactat gctgtcttga acgagagaat gaccaatgac 3240
agtcaaatgg atccaaagcg tgtccggttt aggcgaattt cttaaacttt cttcaactgt 3300
tggcaattta tcagatccat aatctgcaaa gacctttaaa tctcgattat ctttcggaga 3360
tgaacatttg ggaagatact gtttgggtgg tttaaaagcg gtaagataat gtatcctagc 3420
ccgctcgacc aaagaaatgc ttttccaggt actgtggtct aaacgggatc ggaaattacg 3480
catatcttcg atttgagagt aagcaccatc ttcataagcc atagaaatca tctctggtgt 3540
ttcaaactca ttcgaaccat atattttgag acttgagtta atatagtgag aagtaacaga 3600
agatggataa taggtagtag cacgagtcaa gagataatga tcaagggtat cgttttgaat 3660
ttgcttaatg gacatagtgt cgtaaacttt agcagcagca gggaagcctc catcaagaag 3720
aagatataaa cgaataaggg gtagctttaa atgaaagtta tgttgactat aagtaatgcc 3780
tttttctaaa agacagattg cgtcaaaaat taaagcttgt ttttcggcag gttttaaatc 3840
cttattcccc tcccacatat atatcaatga atgaactgct aataacaaag cttcataacc 3900
gtgcgtaaag tcagtgggta ataagccttt acttaaagac aaaccctttt caaatgcgac 3960
gaagcatcta cgcacgtaat cgacaaccga ttctgcagta aacgactcaa agagcaaaaa 4020
atggatcttc agaagcaaaa cctcagcata taatttatcg actttttgag actacgacga 4080
gttaatattg aaagatgaat aaatacttac ttcattggat tcacctagat cagccaactt 4140
aaatgcatct aataaccggt gttgtgcatc aacattcagc ttcaatagat aaggtctcaa 4200
atcttcaaaa acaattggtt tcatatacag ctttttgata tagccaagta atgctgattc 4260
gtgttcttca ggaaaaaacc gtgcgctggc ttcaatccaa agtaaatgaa gatttctttt 4320
tgtgctgctg gttgacaacg ctttcaatat acaatccttt aatgggacta atctatggag 4380
aagttagtaa taatgcaaaa aaaaaaactt actttgaatc atcattggaa gcactatcca 4440
gcaaagcttt gcaaaccttc caatcagtat tcccagtttg gaaaagagaa agcgaaaacg 4500
tgaataaaga atcccaacgc gcacaggaag caagcaattc aagcttcctc aaaagaaggt 4560
cggcgtcagc atcaacaaag cgatcagcat cttgatgtat taaagcatct aacgctctgt 4620
ccttgtctcc cactaaaagc aacacgtcta aataaagatg aaactcctca caagaatcga 4680
tataacctgt tggtttttcg aaaatgagtt tagcagtttt ttcagctagt gctttcagca 4740
aacgttgttc aacctcattt tccgactttt tggacaaaag atacaaagat gagataaccc 4800
ataaggtatg tttcctagaa ggaaagttct tttgcaattc tacagcagcc tgaggagtta 4860
gtaaaaaaaa aacaaattaa gatcttacct ttctttggtg agacaatgat ttgatgcgaa 4920
tagatgcttt gaagtaagct aataagtttt tttcctgctt tccataagtt tgcaagaact 4980
tttcccaaaa tacaaacgat tcttctcctt caaatagtta ataacttaaa agtaagaggg 5040
taaaaacaag tccattgaga aataaaacgt tgcacttgaa tcatcaagct acgaaaagaa 5100
caaagatcgt ttttcttaag caaagtttaa ccgatcaaat aactaaagcc tttatctcaa 5160
ggaaatagta gaatattcaa aataaaaaat acgaccaaag tgctatcaac atttatgttc 5220
atctgttcaa tagaacctaa ttatacttac ccttcttttg atcatcataa acagcttgta 5280
taatgtctaa aagttccaag ctgttgatgg gagtcgattt taacggttct aaaagtgcta 5340
gagcctcggg accacgtcct gcctgagcca aggataacgc tgaataaacc taaaaaggat 5400
gttaacagct aaaaacataa ctcgattact tacaattgtt gattctttac tcccagaacg 5460
acgcattgta gtaggcaatg aaattaaagc ccaaacagta gtcagaagtt ggtaggtgtt 5520
gcagtcaaat tattattcta cagaggagaa tattatagcc agcgtggtag aatctggata 5580
tatatctact gcaaaagtgt aattgcattg gtttaaaggg tatactatgg ttaagtaata 5640
tattcacagc tgtacaattt acagtcataa ctaaaacttc cttaagccgt aaagaaatac 5700
ctggtgttgt aaaatttgtt gtatatccac ggcatggtca tataatgtga ttttgtgctc 5760
aaataaatat aaaatatgca taatttttgt acatttaatt tgagaaaccc atcttttgtt 5820
gagaggctgt caatgaatag cagtttcatt gaaaagcagc gggatgacca gaaaagtatt 5880
ttacaatggc aagggagtag aaagctagcg taatattcag aaagctaggt aattgagcaa 5940
tcctttaatt cattgctaag catgctaggt aaacgcagta aacctttcag ttttcattta 6000
ggtataaggc tgtttaatga gtatctccac taaatttaaa gatcaaaact cagtatcaat 6060
tcttaaaagt tttattttat ttaataatca tatacttctc ataatctttc aattttttcc 6120
ccattttgat gatattttta ttaatcctac agtaagctct atgatatcgt tattcttcaa 6180
ataggctggt cagcacgtgg acggtgttac ttatcgttaa ataaatcgta ctaaggaggt 6240
gcgatgtaaa tgatatgctt gtcaagtatt aactgctctc caccaaccgc cggtttaact 6300
gattattgtt gaaaagcgca gacgaagttt agagaattac tagcgtattt taaatttaat 6360
caacggacta ttttttattc ctttgagatc cgactttatc gctttgcttc taattttcca 6420
aaattcagtc tatctacgcg atccagccct gtttgcgtaa atttcatatt atttttcttt 6480
aaacgtttgg 6490
//