Skip to content

HTTPS clone URL

Subversion checkout URL

You can clone with HTTPS or Subversion.

Download ZIP
branch: master
Fetching contributors…

Cannot retrieve contributors at this time

276 lines (275 sloc) 17.67 kb
LOCUS SPBC1215 6490 bp DNA PLN 16-JUL-1999
DEFINITION S.pombe chromosome II cosmid c1215.
ACCESSION AL096846
VERSION AL096846.1 GI:5531459
KEYWORDS actin cytoskeleton organisation; COX complex biogenesis; DEC1
homologue; mitochondrial inheritance; SURF-family protein.
SOURCE fission yeast.
ORGANISM Schizosaccharomyces pombe
Eukaryota; Fungi; Ascomycota; Schizosaccharomycetes;
Schizosaccharomycetales; Schizosaccharomycetaceae;
Schizosaccharomyces.
REFERENCE 1 (bases 1 to 6490)
AUTHORS Lyne,M.H., Rajandream,M.A., Barrell,B.G., Seeger,K., Quail,M. and
Harris,D.
TITLE Direct Submission
JOURNAL Submitted (16-JUL-1999) European Schizosaccharomyces genome
sequencing project, Sanger Centre, The Wellcome Trust Genome
Campus, Hinxton, Cambridge CB10 1SA, E-mail: barrell@sanger.ac.uk
and
COMMENT Notes:
Details of yeast sequencing at the Sanger Centre are available on
the World Wide Web.
(URL, http://www.sanger.ac.uk/Projects/S_pombe/)
During 1995 to 1996 about 66% of S. pombe chromosome 1 was
sequenced by the Sanger Centre. The sequencing of the S. pombe
genome is now being continued with funding from The European
Commission. Fourteen European sequencing laboratories, including
the Sanger Centre, are participating in the project.
Protein coding regions (CDS) have been predicted with the help of
computer analysis using the Genefinder program in PomBase (an ACEDB
database) with additional predictions for the branch-acceptor sites
supplied by the program Sp3splice. CAUTION: It is possible that for
any individual CDS we may have underestimated or overestimated the
number of introns/exons or we may not have chosen the correct
splice donor/acceptor sites.
CDS are numbered using the following system eg SPBC25H2.01c. SP (S.
pombe), B (chromosome 2), c25H2 (cosmid name), .01 (first CDS), c
(complementary strand).
The more significant matches with motifs in the PROSITE database
are also included but some of these may be fortuitous.
The length in codons is given for each CDS.
IMPORTANT: This sequence MAY NOT be the entire insert of the
sequenced clone. It may be shorter because we only sequence
overlapping sections once, or longer, because we arrange for a
small overlap between neighbouring submissions.
Cosmid c1215 is overlapped at the 5' end by cosmid c1750, EMBL
entry SPAB4534, accession number AB004534, and at the 3' end by
cosmid c83, EMBL entry SPBC83, accession number AL035536.
FEATURES Location/Qualifiers
source 1..6490
/organism="Schizosaccharomyces pombe"
/strain="972h-"
/db_xref="taxon:4896"
/chromosome="II"
/map="IIL"
/clone="cosmid c1215"
misc_feature complement(1..82)
/note="nominal overlap with cosmid SPAB4534 (c1750) S.
pombe chromosome 2"
tRNA 36..107
/note="tRNA Thr anticodon TGT, Cove score 78.51"
/product="tRNA-Thr"
rRNA 415..581
/note="SPRG5SD K00771 Yeast (s.pombe) 5s rrna gene and
flanks"
CDS join(1501..1760,1844..2456)
/gene="SPBC1215.01"
/note="SPBC1215.01, len:290, SIMILARITY:Saccharomyces
cerevisiae, YG2X_YEAST, hypothetical 45.1 kd protein in
clb6-spt6 intergenic region, (389 aa), fasta scores: opt:
385, E():2.8e-19, (29.0% identity in 314 aa)"
/codon_start=1
/label=SPBC1215.01
/product="putative SURF-family protein"
/protein_id="CAB50922.1"
/db_xref="GI:5531460"
/translation="MFWWKSATKFTFSKRGPCVFRYLSTLEGTTVRPKKNKFLVGLLS
AVPIVTFALGTWQVKRREWKMGIINTLTERLQQPAILLPKTVTEQDTKKLEWTRVLLR
GVFCHDQEMLVGPRTKEGQPGYHVVTPFILDDGRRILVNRGWIARSFAEQSSRDPSSL
PKGPVVIEGLLRQHTDKPRFMMKNEPEKNSFYFLNVREFAQLKGTLPILITELQPSLT
PLQEADHVKRGLPLGHPLKVEIFNSHTEYIITWYSLSVVSAIMLYVYFKRGSGTSSLN
SAYERSKILNNKRL"
gene join(1501..1760,1844..2456)
/gene="SPBC1215.01"
misc_feature 1761..1766
/gene="SPBC1215.01"
/note="gtacgt, splice donor sequence"
misc_feature 1827..1843
/gene="SPBC1215.01"
/note="ctaacataatcacttag, splice branch and acceptor"
CDS complement(join(2495..2602,2663..2881,2953..4071,4111..4372,4413..4849,4889..5007,5251..5389,5434..5466))
/gene="SPBC1215.02c"
/note="SPBC1215.02c, len:811, SIMILARITY:Saccharomyces
cerevisiae, DEC1_YEAST, dec1 protein, (796 aa), fasta
scores: opt: 184, E():0.00014, (23.4% identity in 577 aa)"
/codon_start=1
/label=SPBC1215.02c
/product="similar to yeast DEC1 mitochondrial inheritance
and actin cytoskeleton organisation protein"
/protein_id="CAB50923.1"
/db_xref="GI:5531461"
/translation="MRRSGSKESTIVYSALSLAQAGRGPEALALLEPLKSTPINSLEL
LDIIQAVYDDQKKGEESFVFWEKFLQTYGKQEKNLLAYFKASIRIKSLSHQRKAAVEL
QKNFPSRKHTLWVISSLYLLSKKSENEVEQRLLKALAEKTAKLIFEKPTGYIDSCEEF
HLYLDVLLLVGDKDRALDALIHQDADRFVDADADLLLRKLELLASCARWDSLFTFSLS
LFQTGNTDWKVCKALLDSASNDDSKLVPLKDCILKALSTSSTKRNLHLLWIEASARFF
PEEHESALLGYIKKLYMKPIVFEDLRPYLLKLNVDAQHRLLDAFKLADLGESNESQKV
DKLYAEVLLLKIHFLLFESFTAESVVDYVRRCFVAFEKGLSLSKGLLPTDFTHGYEAL
LLAVHSLIYMWEGNKDLKPAEKQALIFDAICLLEKGITYSQHNFHLKLPLIRLYLLLD
GGFPAAAKVYDTMSIKQIQNDTLDHYLLTRATTYYPSSVTSHYINSSLKIYGSNEFET
PEMISMAYEDGAYSQIEDMRNFRSRLDHSTWKSISLVERARIHYLTAFKPPKQYLPKC
SSPKDNRDLKVFADYGSDKLPTVEESLRNSPKPDTLWIHLTVIGHSLVQDSIVNGDFE
KAVLSAKEMEVLCENNDLSKQLTSEEIVHMKLLIQLGLLSVKVKNGDYENSSFETIEN
LIESFDYENSTPLSQLTKYTEIINDLITCLNSFLYHVSATKKKEFTRQYQLLKNISSN
KLGSISGITKHKKKAARKYVSELLSNSWLSNLSETQVPYDPKFAKQVGEGMIDSYIQT
TDAVSKLPKFVKF"
gene complement(join(2495..2602,2663..2881,2953..4071,4111..4372,4413..4849,4889..5007,5251..5389,5434..5466))
/gene="SPBC1215.02c"
misc_feature complement(2603..2612)
/gene="SPBC1215.02c"
/note="ctaataatag, splice branch and acceptor"
misc_feature complement(2657..2662)
/gene="SPBC1215.02c"
/note="gtacgt, splice donor sequence"
misc_feature complement(2882..2896)
/gene="SPBC1215.02c"
/note="ctaaccgaattatag, splice branch and acceptor"
misc_feature complement(2947..2952)
/gene="SPBC1215.02c"
/note="gtaagt, splice donor sequence"
misc_feature complement(4072..4085)
/gene="SPBC1215.02c"
/note="ttaactcgtcgtag, splice branch and acceptor"
misc_feature complement(4105..4110)
/gene="SPBC1215.02c"
/note="gtaagt, splice donor sequence"
misc_feature complement(4373..4387)
/gene="SPBC1215.02c"
/note="ctaacttctccatag, splice branch and acceptor"
misc_feature complement(4407..4412)
/gene="SPBC1215.02c"
/note="gtaagt, splice donor sequence"
misc_feature complement(4850..4861)
/gene="SPBC1215.02c"
/note="ctaactcctcag, splice branch and acceptor"
misc_feature complement(4883..4888)
/gene="SPBC1215.02c"
/note="gtaaga, splice donor sequence"
misc_feature complement(5008..5021)
/gene="SPBC1215.02c"
/note="ttaactatttgaag, splice branch and acceptor"
misc_feature complement(5245..5250)
/gene="SPBC1215.02c"
/note="gtaagt, splice donor sequence"
misc_feature complement(5390..5405)
/gene="SPBC1215.02c"
/note="ttaacatcctttttag, splice branch and acceptor"
misc_feature complement(5428..5433)
/gene="SPBC1215.02c"
/note="gtaagt, splice donor sequence"
misc_feature 6387..6490
/note="nominal overlap with cosmid SPBC83 S. pombe
chromosome 2"
BASE COUNT 0 a 0 c 0 g 0 t
ORIGIN
1 tatatataat ttaataaata cattccgacg atactgcctc tatggcttag tggtacagca
61 tcgcacttgt aatgcgaaga tccttggttc gattccgagt ggaggcatat acattatatt
121 atattctttt tcatgcggaa aaaagatttc aaatttttgg gtatgatatt aatatgactg
181 taacgttaat agcaaagtga gtgttaataa tgataaaata gcagcaaaat ctcttttccg
241 agtaagacgt tttccagtct aaatttggag tctgcagttg tttcgcaatt cttaatgtat
301 ggttatacta aatacaaact ttaaagctct gatttatgtt tgcaataaac taaaataaaa
361 gcacaaaaac ctttacccat taatttcaaa caacttataa actaccggta aacttttttt
421 ctaaccttta taatttataa actagaatgt ttaatgtcta cggccatacc taggcgaaaa
481 caccagttcc cgtccgatca ctgcagttaa gcgtctgagg gcctcgttag tactatggtt
541 ggagacaaca tgggaatccg gggtgctgta ggctattttt ttatatccgt ctttcttact
601 acttgcctaa caagtcatga tgtactctca aaatatgttt gcatgccttg taatattggt
661 tatggatagc tccttctgga cttgatcttt tgtagccaag aacaatgggt atagactctg
721 accttgtgat gttgtagcca cagattataa taggtatttt caagtacagt aacaaaaatc
781 ttctagtttt tttttagaaa ggatacacca agtataagca aattcaggaa ttgttgatta
841 aactgtcaac ttcggtaaaa ctttgggcat aagtagtgtg ggagcaagtt taactaaaat
901 tctattcaga tgtcgaatcc aaaccgctaa ttttgctcaa ctagcttttc ataaaaacca
961 attcatagtt tcatactaat aaagacgatt gtttacttta aaacatacgt cgtaagaaca
1021 tatattgctt tatcgaaaga taacaaatgt taagctatta tattatttaa ctatagcgca
1081 gatttcgctt cctttactta aaaaagacat gtgacttgta gaagcttgga gtgaatacgc
1141 aaaggtacct acttagacat tcgcgtctct cttagctgtc aacatcaaca aactggcccc
1201 gtattgaaca gtatcttact tgtcgaagga tttgactaag aaaattttat ttctttatag
1261 caatattccg ttttcgctta gaagattcta gtcaattgcc ctattctact tacgctttac
1321 agtagtatca gaagacctga gtgggatttt gctgctagta gaggccattc aagttaactc
1381 cgttttgcaa cattttaaaa gtttttgaat tgaatataaa tatcaattgt ttgattcctt
1441 ttaggattta atcttttctt tttatttttg tttcgattga atcttggatt cctgtctatc
1501 atgttttggt ggaaaagtgc tactaaattc acattctcaa agcgtggacc gtgtgtcttt
1561 cgctatttga gtactcttga aggaacaact gtgaggccta aaaaaaataa atttttagtt
1621 ggattgcttt ctgccgttcc aattgtcacg tttgctttag gaacttggca ggtaaagcga
1681 cgagaatgga aaatgggtat catcaataca ctcacggaaa ggcttcaaca gcccgcaatt
1741 ttattaccga aaactgttac gtacgttaag ttaacatata cacaaattgc acgttttgca
1801 attgaactgt cgttttttac attaagctaa cataatcact tagagagcaa gatacaaaaa
1861 aacttgagtg gactagggtt ttgcttcgtg gtgtgttttg tcacgaccaa gaaatgttgg
1921 tcggtccaag aacgaaggaa ggccaacctg gctatcacgt agtaacccca tttattttag
1981 acgatgggcg tcgaatttta gtcaacagag gatggattgc tcgatcattt gctgaacagt
2041 cttctcgaga tcctagttct ttacctaaag gtccagtggt cattgaaggt cttttgagac
2101 aacatactga taagccaaga tttatgatga agaatgagcc tgaaaaaaat tctttttact
2161 tcttaaatgt tcgtgagttt gcacaattga aaggaactct ccccattttg ataacagaac
2221 tacaaccatc gcttacaccg ttgcaagaag ccgatcatgt taagagaggc ttgcctcttg
2281 gtcatcctct aaaagttgaa attttcaaca gtcatacaga atatattatc acttggtatt
2341 ctctaagtgt ggtatcagct ataatgcttt acgtctattt taagagaggt tcaggcacat
2401 cttctctgaa ttctgcatac gaaagaagca agattctaaa caacaaacga ttataaaaaa
2461 ttttcatatt tataagtttc taaatattat ctacctaaaa ttttacaaat tttggaagct
2521 tgcttactgc gtccgtcgtt tgaatgtatg aatcgatcat tccttcacca acttgttttg
2581 caaacttcgg atcgtaagga acctattatt aggaagttag ttctcatgcc tataatttta
2641 atgctctata atcatcacgt acctgagttt cagataaatt gctcagccaa gagtttgaca
2701 acaattctga tacatatttt ctggcggcct tctttttatg cttcgtaatt ccagagatac
2761 ttcctaattt gttactgctg atgtttttaa gaagttggta ttgtctggta aattcttttt
2821 tcttggtagc tgacacgtga tataggaagc tgtttagaca agtgataagg tcatttatga
2881 tctataattc ggttagttta gaaattttta tatcattttt tttaaaaaaa aaaaccagtt
2941 tggtaaactt acttcagtgt actttgtcaa ttgacttaaa ggcgtggagt tctcataatc
3001 gaatgactca attaaatttt caatcgtttc gaacgaggaa ttttcgtagt ctccgttctt
3061 gacctttacc gaaagtaatc ccagttggat caataatttc atatgaacaa tttcttccga
3121 agttaattgc ttcgataaat cattgttttc gcacaaaact tccatttcct tagcacttaa
3181 aacggccttt tcaaaatcgc cgtttactat gctgtcttga acgagagaat gaccaatgac
3241 agtcaaatgg atccaaagcg tgtccggttt aggcgaattt cttaaacttt cttcaactgt
3301 tggcaattta tcagatccat aatctgcaaa gacctttaaa tctcgattat ctttcggaga
3361 tgaacatttg ggaagatact gtttgggtgg tttaaaagcg gtaagataat gtatcctagc
3421 ccgctcgacc aaagaaatgc ttttccaggt actgtggtct aaacgggatc ggaaattacg
3481 catatcttcg atttgagagt aagcaccatc ttcataagcc atagaaatca tctctggtgt
3541 ttcaaactca ttcgaaccat atattttgag acttgagtta atatagtgag aagtaacaga
3601 agatggataa taggtagtag cacgagtcaa gagataatga tcaagggtat cgttttgaat
3661 ttgcttaatg gacatagtgt cgtaaacttt agcagcagca gggaagcctc catcaagaag
3721 aagatataaa cgaataaggg gtagctttaa atgaaagtta tgttgactat aagtaatgcc
3781 tttttctaaa agacagattg cgtcaaaaat taaagcttgt ttttcggcag gttttaaatc
3841 cttattcccc tcccacatat atatcaatga atgaactgct aataacaaag cttcataacc
3901 gtgcgtaaag tcagtgggta ataagccttt acttaaagac aaaccctttt caaatgcgac
3961 gaagcatcta cgcacgtaat cgacaaccga ttctgcagta aacgactcaa agagcaaaaa
4021 atggatcttc agaagcaaaa cctcagcata taatttatcg actttttgag actacgacga
4081 gttaatattg aaagatgaat aaatacttac ttcattggat tcacctagat cagccaactt
4141 aaatgcatct aataaccggt gttgtgcatc aacattcagc ttcaatagat aaggtctcaa
4201 atcttcaaaa acaattggtt tcatatacag ctttttgata tagccaagta atgctgattc
4261 gtgttcttca ggaaaaaacc gtgcgctggc ttcaatccaa agtaaatgaa gatttctttt
4321 tgtgctgctg gttgacaacg ctttcaatat acaatccttt aatgggacta atctatggag
4381 aagttagtaa taatgcaaaa aaaaaaactt actttgaatc atcattggaa gcactatcca
4441 gcaaagcttt gcaaaccttc caatcagtat tcccagtttg gaaaagagaa agcgaaaacg
4501 tgaataaaga atcccaacgc gcacaggaag caagcaattc aagcttcctc aaaagaaggt
4561 cggcgtcagc atcaacaaag cgatcagcat cttgatgtat taaagcatct aacgctctgt
4621 ccttgtctcc cactaaaagc aacacgtcta aataaagatg aaactcctca caagaatcga
4681 tataacctgt tggtttttcg aaaatgagtt tagcagtttt ttcagctagt gctttcagca
4741 aacgttgttc aacctcattt tccgactttt tggacaaaag atacaaagat gagataaccc
4801 ataaggtatg tttcctagaa ggaaagttct tttgcaattc tacagcagcc tgaggagtta
4861 gtaaaaaaaa aacaaattaa gatcttacct ttctttggtg agacaatgat ttgatgcgaa
4921 tagatgcttt gaagtaagct aataagtttt tttcctgctt tccataagtt tgcaagaact
4981 tttcccaaaa tacaaacgat tcttctcctt caaatagtta ataacttaaa agtaagaggg
5041 taaaaacaag tccattgaga aataaaacgt tgcacttgaa tcatcaagct acgaaaagaa
5101 caaagatcgt ttttcttaag caaagtttaa ccgatcaaat aactaaagcc tttatctcaa
5161 ggaaatagta gaatattcaa aataaaaaat acgaccaaag tgctatcaac atttatgttc
5221 atctgttcaa tagaacctaa ttatacttac ccttcttttg atcatcataa acagcttgta
5281 taatgtctaa aagttccaag ctgttgatgg gagtcgattt taacggttct aaaagtgcta
5341 gagcctcggg accacgtcct gcctgagcca aggataacgc tgaataaacc taaaaaggat
5401 gttaacagct aaaaacataa ctcgattact tacaattgtt gattctttac tcccagaacg
5461 acgcattgta gtaggcaatg aaattaaagc ccaaacagta gtcagaagtt ggtaggtgtt
5521 gcagtcaaat tattattcta cagaggagaa tattatagcc agcgtggtag aatctggata
5581 tatatctact gcaaaagtgt aattgcattg gtttaaaggg tatactatgg ttaagtaata
5641 tattcacagc tgtacaattt acagtcataa ctaaaacttc cttaagccgt aaagaaatac
5701 ctggtgttgt aaaatttgtt gtatatccac ggcatggtca tataatgtga ttttgtgctc
5761 aaataaatat aaaatatgca taatttttgt acatttaatt tgagaaaccc atcttttgtt
5821 gagaggctgt caatgaatag cagtttcatt gaaaagcagc gggatgacca gaaaagtatt
5881 ttacaatggc aagggagtag aaagctagcg taatattcag aaagctaggt aattgagcaa
5941 tcctttaatt cattgctaag catgctaggt aaacgcagta aacctttcag ttttcattta
6001 ggtataaggc tgtttaatga gtatctccac taaatttaaa gatcaaaact cagtatcaat
6061 tcttaaaagt tttattttat ttaataatca tatacttctc ataatctttc aattttttcc
6121 ccattttgat gatattttta ttaatcctac agtaagctct atgatatcgt tattcttcaa
6181 ataggctggt cagcacgtgg acggtgttac ttatcgttaa ataaatcgta ctaaggaggt
6241 gcgatgtaaa tgatatgctt gtcaagtatt aactgctctc caccaaccgc cggtttaact
6301 gattattgtt gaaaagcgca gacgaagttt agagaattac tagcgtattt taaatttaat
6361 caacggacta ttttttattc ctttgagatc cgactttatc gctttgcttc taattttcca
6421 aaattcagtc tatctacgcg atccagccct gtttgcgtaa atttcatatt atttttcttt
6481 aaacgtttgg
//
Jump to Line
Something went wrong with that request. Please try again.