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INTRODUCTION Artemis is a free genome browser and annotation tool that allows visualisation of sequence features, next generation data and the results of analyses within the context of the sequence, and also its six-frame translation. Artemis is written in Java, and is available for UNIX, Macintosh and Windows systems. It can read EMBL and GENBANK database entries or sequence in FASTA, indexed FASTA or raw format. Other sequence features can be in EMBL, GENBANK or GFF format. ACT is a free tool for displaying pairwise comparisons between two or more DNA sequences. It can be used to identify and analyse regions of similarity and difference between genomes and to explore conservation of synteny, in the context of the entire sequences and their annotation. DOCUMENTATION The Artemis user manual is at: http://www.sanger.ac.uk/resources/software/artemis/ The ACT user manual is at: http://www.sanger.ac.uk/resources/software/act/ INSTALLATION The installation instructions are included in the user manual. DISTRIBUTION Artemis may be freely distributed under the terms of the GNU Public License, and should run on any system with a recent version of Java. For information on how to get Artemis see this web page: http://www.sanger.ac.uk/resources/software/artemis/ COPYRIGHT Copyright (C) 1998-2011 Genome Research Limited This program is free software; you can redistribute it and/or modify it under the terms of the GNU General Public License as published by the Free Software Foundation; either version 2 of the License, or (at your option) any later version. This program is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details. You should have received a copy of the GNU General Public License along with this program; if not, write to the Free Software Foundation, Inc., 59 Temple Place - Suite 330, Boston, MA 02111-1307, USA.