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#!/usr/bin/env perl
# From Torsten Seemann commit f46312e9df539c56b058f0ef25479d7297ceec89
# https://raw.githubusercontent.com/tseemann/nullarbor/master/bin/roary2svg.pl
use warnings;
use strict;
use Data::Dumper;
use List::Util qw(min max sum);
use List::MoreUtils qw(uniq all any);
use Text::CSV;
use SVG;
use constant FONT_ASPECT => 0.8;
my(@Options, $verbose, $taxacol, $width, $height, $acconly,
$consensus, $border, $colour, $sepcolour);
setOptions();
# read gene_presence_absence.csv from stdin
# "Gene","Non-unique Gene name","Annotation","No. isolates","No. sequences","Avg sequences per isolate","Genome Fragment","Order within Fragment","Accessory Fragment","Accessory Order with Fragment","QC","SRR2352235","SRR2352236","SRR2352237","SRR2352238","SRR2352239","SRR2352240","SRR2352241","SRR2352242","SRR2352243","SRR2352244","SRR2352245","SRR2352246","SRR2352247","SRR2352248","SRR2352249","SRR2352250","SRR2352251","SRR2352252"
my $csv = Text::CSV->new() or die $!;
my $count=0;
my @matrix;
my @id;
my $N;
my $C=0;
my @tally; # genes per taxon
my @is_core; # boolean for this cluster being core
while (my $row = $csv->getline(\*ARGV) ) {
if ($count == 0) {
@id = splice @$row, $taxacol;
$N = scalar(@id);
print STDERR "Found $N taxa: @id\n";
}
else {
my @present = map { $row->[$taxacol+$_] ? 1 : 0 } (0 .. $N-1);
my $num_present = sum(@present);
$is_core[$count] = ($num_present == $N);
next if $acconly and $is_core[$count];
# next if $panonly and all { $_==1 } @present;
push @{ $matrix[$_] }, $present[$_] for (0 .. $N-1);
$tally[$_] += $present[$_] for (0 .. $N-1);
$C++;
}
$count++;
}
print STDERR "Found $C clusters.\n";
my $real_height = $height*($N+1);
my $svg = SVG->new(width=>$width, height=>$real_height);
my $dy = $height;
my $fontsize = 0.75 * $dy;
my $lchars = max( map { length($_) } @id );
my $llen = $fontsize * (1 + $lchars) * FONT_ASPECT;
my $rchars = max( map { length("$_") } @tally);
my $rlen = $fontsize * (1 + $rchars) * FONT_ASPECT;
my $width2 = $width - $llen - $rlen;
my $dx = $width2 / $C;
my $font_style = { 'font-family'=>'sans-serif', 'fill'=>'black', 'font-size'=>$fontsize };
print STDERR "Box = $dx x $dy px\n";
print STDERR "Left label = $lchars chr x $fontsize px\n";
print STDERR "Right label = $rchars chr x $fontsize px\n";
for my $j (0 .. $N-1) {
for my $i (0 .. $C-1) {
# print STDERR "$j $i $matrix[$j][$i]\n";
if ($matrix[$j][$i]) {
# box for each present gene
$svg->rectangle(
'x' => $llen+$i*$dx, 'y' => $j*$dy, 'width' => $dx, 'height' => $dy-1,
'style' => { fill=>$colour, opacity=>($is_core[$i] ? 1 : 0.75) },
);
}
}
# taxon label for each row
$svg->text( x=>$fontsize, y=>($j+0.75)*$dy, -cdata=>$id[$j], style=>$font_style );
# number of genes for each row
$svg->text( x=>$llen+$width2+$fontsize, y=>($j+0.75)*$dy, -cdata=>$tally[$j], style=>$font_style );
# separator line
my $ypos = ($j+1)*$dy;
$svg->line( x1=>0, y1=>$ypos, x2=>$width, y2=>$ypos, style=>{stroke=>$sepcolour});
}
# bottom label
my $bottom_text = "$N taxa, $C clusters";
$bottom_text .= $acconly ? " (accessory only)" : " (core + accessory)";
$svg->text( x=>$llen, y=>($N+0.75)*$dy, -cdata=>$bottom_text, style=>$font_style );
# border
if ($border) {
$svg->rectangle(
'x' => 0, 'y' => 0, 'width' => $width, 'height' => $real_height,
'style' => { stroke=>$sepcolour, fill=>'none' },
);
}
print STDERR "Writing SVG file\n";
print STDOUT $svg->xmlify;
print STDERR "Done.\n";
#----------------------------------------------------------------------
# Option setting routines
sub setOptions {
use Getopt::Long;
@Options = (
{OPT=>"help", VAR=>\&usage, DESC=>"This help"},
{OPT=>"verbose!", VAR=>\$verbose, DEFAULT=>0, DESC=>"Verbose output"},
{OPT=>"width=i", VAR=>\$width, DEFAULT=>1024, DESC=>"Canvas width"},
{OPT=>"height=i", VAR=>\$height, DEFAULT=>20, DESC=>"Row height"},
{OPT=>"taxacolumn=i", VAR=>\$taxacol, DEFAULT=>14, DESC=>"Column in gpa.csv where taxa begin"},
{OPT=>"colour=s", VAR=>\$colour, DEFAULT=>'DimGray', DESC=>"Colour of core cells"},
{OPT=>"sepcolour=s", VAR=>\$sepcolour, DEFAULT=>'LightGray', DESC=>"Colour of horizontal separators/borders"},
{OPT=>"acconly!", VAR=>\$acconly, DEFAULT=>0, DESC=>"Only draw accessory (non-core) genes"},
# {OPT=>"consensus!", VAR=>\$consensus, DEFAULT=>0, DESC=>"Add consensus row"},
{OPT=>"border!", VAR=>\$border, DEFAULT=>0, DESC=>"Add outline border"},
);
(!@ARGV) && (usage());
&GetOptions(map {$_->{OPT}, $_->{VAR}} @Options) || usage();
# Now setup default values.
foreach (@Options) {
if (defined($_->{DEFAULT}) && !defined(${$_->{VAR}})) {
${$_->{VAR}} = $_->{DEFAULT};
}
}
}
sub usage {
print "Usage: $0 [options] gene_presence_absence.csv > pan_genome.svg\n";
foreach (@Options) {
printf " --%-13s %s%s.\n",$_->{OPT},$_->{DESC},
defined($_->{DEFAULT}) ? " (default '$_->{DEFAULT}')" : "";
}
exit(1);
}
#----------------------------------------------------------------------