NYSBC - 3DFSC plugin
This plugin provide a wrapper around 3DFSC program developed at Salk Institute and NYSBC.
You will need to use 2.0 version of Scipion to be able to run these protocols. To install the plugin, you have two options:
- Stable version
scipion installp -p scipion-em-nysbc
- download repository
git clone https://github.com/scipion-em/scipion-em-nysbc.git
scipion installp -p path_to_scipion-em-nysbc --devel
3DFSC binaries will be installed automatically with the plugin, but you can also
link an existing installation. Important: you need to have conda
(miniconda3 or anaconda3) pre-installed to use this program.
Default installation path assumed is
software/em/nysbc3DFSC-2.5, if you want
to change it, set NYSBC_3DFSC_HOME in
scipion.conf file to the folder where
the 3DFSC is installed.
To check the installation, simply run the following Scipion test:
scipion test nysbc.tests.test_protocols_nysbc.TestNysbc3DFSC
- 3d fsc
- Yong Zi Tan, Philip R Baldwin, Joseph H Davis, James R Williamson, Clinton S Potter, Bridget Carragher & Dmitry Lyumkis. Addressing preferred specimen orientation in single-particle cryo-EM through tilting. Nature Methods volume 14, pages 793–796 (2017).