diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index 294414cb..ed16eedd 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -11,10 +11,11 @@ on:
 
 jobs:
   test:
-    runs-on: ubuntu-latest
+    runs-on: ${{ matrix.os }}
     strategy:
       matrix:
         java: [ '8', '17' ]
+        os: [ 'ubuntu-latest' ]
 
     steps:
       - name: checkout the repo
diff --git a/src/main/scala/scoverage/ScoverageSbtPlugin.scala b/src/main/scala/scoverage/ScoverageSbtPlugin.scala
index 57ca108c..d59dbf91 100644
--- a/src/main/scala/scoverage/ScoverageSbtPlugin.scala
+++ b/src/main/scala/scoverage/ScoverageSbtPlugin.scala
@@ -2,6 +2,7 @@ package scoverage
 
 import sbt.Keys._
 import sbt._
+import sbt.internal.util.Util.isWindows
 import sbt.plugins.JvmPlugin
 import scoverage.reporter.CoberturaXmlWriter
 import scoverage.domain.Constants
@@ -147,7 +148,7 @@ object ScoverageSbtPlugin extends AutoPlugin {
 
         Seq(
           Some(
-            s"-Xplugin:${pluginPaths.mkString(":")}"
+            s"-Xplugin:${pluginPaths.mkString(java.io.File.pathSeparator)}"
           ),
           Some(
             s"-P:scoverage:dataDir:${coverageDataDir.value.getAbsolutePath}/scoverage-data"
@@ -160,6 +161,13 @@ object ScoverageSbtPlugin extends AutoPlugin {
             .map(v => s"-P:scoverage:excludedPackages:$v"),
           Option(coverageExcludedFiles.value.trim)
             .filter(_.nonEmpty)
+            .map(v =>
+              // On windows, replace unix file separators with windows file
+              // separators. Note that we need to replace / with \\ because
+              // the plugin treats this string as a regular expression and
+              // backslashes must be escaped in regular expressions.
+              if (isWindows) v.replace("/", """\\""") else v
+            )
             .map(v => s"-P:scoverage:excludedFiles:$v"),
           Some("-P:scoverage:reportTestName"),
           // rangepos is broken in some releases of scala so option to turn it off
diff --git a/src/sbt-test/scoverage/coverage-excluded-files/build.sbt b/src/sbt-test/scoverage/coverage-excluded-files/build.sbt
index 22856a91..4fcc343e 100644
--- a/src/sbt-test/scoverage/coverage-excluded-files/build.sbt
+++ b/src/sbt-test/scoverage/coverage-excluded-files/build.sbt
@@ -4,7 +4,7 @@ scalaVersion := "2.13.6"
 
 libraryDependencies += "org.scalameta" %% "munit" % "0.7.29" % Test
 
-coverageExcludedFiles := ".*\\/two\\/GoodCoverage"
+coverageExcludedFiles := ".*/two/GoodCoverage"
 
 resolvers ++= {
   if (sys.props.get("plugin.version").exists(_.endsWith("-SNAPSHOT")))
diff --git a/src/sbt-test/scoverage/scala3-coverage-excluded-files/build.sbt b/src/sbt-test/scoverage/scala3-coverage-excluded-files/build.sbt
index b6fed517..939f7489 100644
--- a/src/sbt-test/scoverage/scala3-coverage-excluded-files/build.sbt
+++ b/src/sbt-test/scoverage/scala3-coverage-excluded-files/build.sbt
@@ -4,7 +4,7 @@ scalaVersion := "3.2.0-RC1"
 
 libraryDependencies += "org.scalameta" %% "munit" % "0.7.29" % Test
 
-coverageExcludedFiles := ".*\\/two\\/GoodCoverage"
+coverageExcludedFiles := ".*/two/GoodCoverage"
 
 resolvers ++= {
   if (sys.props.get("plugin.version").exists(_.endsWith("-SNAPSHOT")))