I'm not going to clean my room again.
Why don't you want to clean your room again?
I already cleaned it somewhat and my mother wants me to do it again.
It seems unusual that she'd ask you to clean a room you've already cleaned. Are there specific issues she's mentioned that need more attention?
No, she's just being extreme in how clean she wants my room. It's my room, and I'm not doing it again.
I can understand your frustration. If it's her house, though, you have a responsibility to keep your space up to her standards of cleanliness. You might ask her what, specifically, she objects to, so you know where to focus your efforts.