Make sure to install the HH-Suite3 and MMseqs2 and adjust the paths in
Also make sure that
awk, tar, pigz, cstranslate_mpi, sed, md5deep, clustalo, kalign, timeout are all installed and available in PATH.
To build your own databases based on the uniclust pipeline you can use the following three scripts:
run_main.sh: Run Main does the clustering, builds the
uniclust30/50/90and does the sequence enrichment of the
run_hhdatabase.sh: Builds the
run_annotate.sh: Builds the annotation files
Make sure to run the scripts in this order.
The pipeline was custom build for out LSF cluster computing environment and can be submitted to the LSF with
bsub < run_mpi.sh for example.
Please adjust the LSF parameters at the beginning of the
run_ scripts. The pipeline assumes a shared file system between the computing nodes.
Webserver & Downloads
We provide a webserver and the Uniclust based on the UniProtKB on https://uniclust.mmseqs.com/.