diff --git a/installers/Windows/req-extras-pull-request.txt b/installers/Windows/req-extras-pull-request.txt
index 35a7b13d8fc..ee68aceeb3c 100644
--- a/installers/Windows/req-extras-pull-request.txt
+++ b/installers/Windows/req-extras-pull-request.txt
@@ -22,9 +22,5 @@ sympy
 ./external-deps/spyder-kernels
 ./external-deps/qdarkstyle
 
-# Dependencies without wheels in PyPI
-# Rtree
-./installers/Windows/assets/rtree/Rtree-0.9.4-cp37-cp37m-win_amd64.whl
-
 # There are no wheels for version 3.3
 cryptography==3.2.1
diff --git a/installers/Windows/req-extras-release.txt b/installers/Windows/req-extras-release.txt
index 4ec63286a14..e0dd964ff63 100644
--- a/installers/Windows/req-extras-release.txt
+++ b/installers/Windows/req-extras-release.txt
@@ -18,9 +18,5 @@ matplotlib
 cython
 sympy
 
-# Dependencies without wheels in PyPI
-# Rtree
-./installers/Windows/assets/rtree/Rtree-0.9.4-cp37-cp37m-win_amd64.whl
-
 # There are no wheels for version 3.3
 cryptography==3.2.1
diff --git a/installers/Windows/req-pull-request.txt b/installers/Windows/req-pull-request.txt
index 87cc3389d45..fb042c7cd28 100644
--- a/installers/Windows/req-pull-request.txt
+++ b/installers/Windows/req-pull-request.txt
@@ -2,8 +2,5 @@
 ./external-deps/spyder-kernels
 ./external-deps/qdarkstyle
 
-# Rtree from external wheel
-./installers/Windows/assets/rtree/Rtree-0.9.4-cp37-cp37m-win_amd64.whl
-
 # There are no wheels for version 3.3
 cryptography==3.2.1
diff --git a/installers/Windows/req-release.txt b/installers/Windows/req-release.txt
index 125956df4dd..9f9533086be 100644
--- a/installers/Windows/req-release.txt
+++ b/installers/Windows/req-release.txt
@@ -1,6 +1,2 @@
-# Dependencies without wheels in PyPI
-# Rtree
-./installers/Windows/assets/rtree/Rtree-0.9.4-cp37-cp37m-win_amd64.whl
-
 # There are no wheels for version 3.3
 cryptography==3.2.1