This package contains a Bioconductor ExpressionSet from Trapnell et al. (2014) paper that performed a time-series experiment of bulk (three bulk samples per time point) and single cell RNA-Seq at four time points (hour 0, 24, 48, 72) in differentiated primary human myoblasts (PMID: 24658644). Metadata and pre-processed data (FPKM) were downloaded from Gene Expression Omnibus (GSE52529). Batch information was extracted from headers of FASTQ files downloaded from Sequence Read Archive.
The R-package trapnell2014myoblasthuman can be installed from Github using the R package devtools.
The data is provided as a
ExpressionSet object can be loaded
by running the following code in R:
library(trapnell2014myoblasthuman) data(trapnell2014myoblasthuman) # Get the expression data eset = exprs(trapnell2014myoblasthuman) # Get the pheno data pd = pData(trapnell2014myoblasthuman)
Report bugs as issues on the GitHub repository