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Brassinosteroid gene regulatory networks at cellular resolution in the Arabidopsis root

This repository contains the code to reproduce the analysis described in Brassinosteroid gene regulatory networks at cellular resolution in the Arabidopsis root

Arabidopsis Root Virtual Expression eXplorer (ARVEX)

Visit ARVEX to interactively view the data associated with this study.

Data

Raw and processed scRNA-seq data associated with this study are available on GEO: GSE212230

input files for the notebooks below are under data/. Note that files larger than github's size limit can be downloaded from ARVEX.

Notebooks

01-Run_COPILOT_preprocessing

02-WT_atlas_dev_stage_reannotation

03-Gene_ontology_WT_atlas_updated_dev_anno

04-Label_transfer

05-Integration

06-Differential_expression_analysis_BL_2hour_vs_BRZ

06-Differential_expression_analysis_bri1-T_vs_WT

06-Differential_expression_analysis_BRZ_vs_Control

06-Differential_expression_analysis_pGL2-BRI1-GFP-bri1-T_vs_WT

06-Differential_expression_analysis_pGL2-BRI1-GFP-bri1-T_vs_bri1-T

06-Differential_expression_analysis_gtl1df1_vs_WT

06-Differential_expression_analysis_gtl1_vs_WT

06-Differential_expression_analysis_df1_vs_WT

07-Prepare_base_GRN_01

07-Prepare_base_GRN_02

07-Prepare_base_GRN_03

07-Prepare_base_GRN_04

08-Run_CellOracle_BR

08-Run_CellOracle_WT

08-CellOracle_WT_GRNs

08-CellOracle_BR_GRNs

08-CellOracle_compare_GRNs

09-WOT_compute_trajectories

09-WOT_analyze_results

10-Compare_BES1_and_GTL1_targets_and_overlap_with_BL_scRNA-seq_data

11-Plot_bri1-T_DEGs_results_on_umap

11-Plot_bri1-T_DEGs

11-Plot_gtl1_df1_DEGs

12-Plot_CellOracle_base_GRNs

12-Plot_CellOracle_BR_cortex_GRN

13-Plot_MDS

14-Plot_163_core_BR_DEGs

15-Plot_cell_length

16-Plot_gtl_df1_samples_on_umap

17-Plots_on_ARVEX

18-Plot_marker_dot_plot

19-Plot_tradeseq

20-Plot_WOT_Cell_wall_signature_over_BR_time_series