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When using Trackmate to track fluorescent particles, the output results sent to XML do not make sense, despite being checked over several raw fluorescence datasets. The displacements between timepoints for the X and Z components of the tracks appear approximately gaussian-distributed, as I would expect, but the Y components are concentrated in small peaks centered on integral pixel displacements. Is it possible to make sure that the particle detection and output generation for these two components are the same?
My pixel dimensions are isotropic, I am using the LoG detector option (no median filtering, subpixel localization=on), and the LAP tracker (no gap closing).
I have run this with the same result on both processed and unprocessed image stacks, so I know this is not an artifact of the image processing.
The text was updated successfully, but these errors were encountered:
I have been in contact with the user and could confirm it on her data.
Using synthetic 2D data, I could not reproduce it. It seems like it happens for 3D solely.
I did quite some extensive tests on it and could not corner the reason behind this bug properly yet. I copy below the state of our discussions.
I guess the problem is not with the LoG detector itself (that's the part that generates integer pixels) but the subpixel localizer:
More precisely, the class called at this line
From Fiji BugZilla issue #1165:
The text was updated successfully, but these errors were encountered: