The code has only been tested with Fluid, but should work in Safari and other WebKit-based browsers (including the iPhone I guess).
Put all Twoot files in a folder somewhere. Download Fluid and create the Twoot.app by following the instructions here:
Launching the app will prompt you for your username and password on the first run.
The default icon was found at http://www.iconarchive.com/show/dragon-soft-icons-by-artua/User-icon.html and is used according to the restrictions described (free for non-commercial use).
The styles/default/Morpho-Helena-128x128.png icon is created by Adrian Melsha and is used in accordance with the license terms here: http://www.iconarchive.com/category/animals/morpho-butterfly-icons-by-adrian-melsha.html
The idea is that you should adapt Twoot to fit your requirements. If you know HTML and CSS it is trivial to add a new style. Create a new folder under the style directory and add all style files (css/images) there. Don't forget to change the stylesheet link in the twoot.htm file.
Currently Twoot has two themes, "Default" and "HUD-black" to match the window styles available in Fluid. To switch theme, edit twoot.htm and point the stylesheet to the correct css file in the styles folder.
Bugs, praise, feature requests
Hit me up with an email at eatsleepgolf at gmail dot com or twitter @travisjeffery.
Create a comment on the initial announcement page.