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Update B cell lecture

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sarahCobey committed Jul 26, 2017
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@@ -124,11 +124,6 @@ <h3>Affinity maturation</h3>
<img class="stretch" src="images/gc.png">
</section>
<section>
<h3>Affinity maturation</h3>
<img class="stretch" src="images/nk_epitopes.png">
</section>
<section>
<h3>Dynamics in one GC</h3>
<img class="stretch" src="images/gc_dynamics.png">
@@ -138,6 +133,16 @@ <h3>Dynamics in one GC</h3>
</a>
</div>
</section>
<section>
<h3>GC B cells evolve</h3>
<img class="stretch" src="images/gc_micro.png">
<div class="citation">
<a href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2119370/pdf/je1763679.pdf">
Jacob and Kelsoe 1992
</a>
</div>
</section>
<section>
<h3>AID induces somatic hypermutation</h3>
@@ -150,24 +155,15 @@ <h3>AID induces somatic hypermutation</h3>
</section>
<section>
<h3>Mutation rate varies over BCR</h3>
<h3>Mutation rate varies over the variable region</h3>
<img class="stretch" src="images/mutations_bcr.jpg">
<div class="citation">
<a href="http://www.pnas.org/content/112/7/E728.full">
Wei et al. 2015
</a>
</div>
</section>
<section>
<h3>GC B cells evolve</h3>
<img class="stretch" src="images/gc_micro.png">
<div class="citation">
<a href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2119370/pdf/je1763679.pdf">
Jacob and Kelsoe 1992
</a>
</div>
</section>
<section>
<h3>GCs produce plasma and memory cells</h3>
@@ -180,7 +176,7 @@ <h3>GCs produce plasma and memory cells</h3>
</section>
<section>
<h3>Memory cells can affinity mature</h3>
<h3>Memory cells can evolve further</h3>
<img src="images/andrews_memory.jpg" style="width: 70%; height: 70%;">
<div class="citation">
<a href="http://stm.sciencemag.org/content/7/316/316ra192">
@@ -354,6 +350,19 @@ <h3>"Antigenic seniority"</h3>
<section data-background="#99CCFF">
<h3>What's so special about early infections?</h3>
</section>
<section>
<h3>A general model of sin</h3>
<img src="images/oas_schematic.png">
<div class="citation">
<a href="http://dx.doi.org/10.1016/j.coviro.2016.12.004">
Cobey and Hensley 2017
</a>
</div>
</section>
<section>
<h3>Response to 2009 H1N1 vaccine</h3>
@@ -402,6 +411,69 @@ <h3>Potential factors influencing specific antibody responses</h3>
<p>Immunogenicity
<p>Autoreactivity
</section>
<section>
<h3>In U.S., flu vaccine coverage is high</h3>
<img src="images/vaccine_coverage.png" style="width: 60%; height: 60%;">
<div class="citation">
<a href="https://www.cdc.gov/flu/fluvaxview/coverage-1516estimates.htm">
FluVaxView
</a>
</div>
</section>
<section>
<h3>In RCT, vaccine provides high indirect protection</h3>
<img src="images/hutterite.png" style="width: 90%; height: 90%;">
<div class="citation">
<a href="http://jamanetwork.com/journals/jama/fullarticle/185509">
Loeb et al. 2010
</a>
</div>
</section>
<section>
<h3>Influenza vaccine effectiveness is low</h3>
<p>H3N2: 33% (95% CI: 26-39)
<p>B: 54% (46-61)
<p>pH1N1: 61% (57-65)
<p>H1N1: 67% (29-85)
<div class="citation">
<a href="http://www.thelancet.com/journals/laninf/article/PIIS1473-3099(16)00129-8/abstract">
Belongia et al. 2016
</a>
</div>
</section>
<section>
<h3>What's going on?</h3>
<p>Poor strain selection (antigenic mismatch)
<p>Egg adaptations (antigenic mismatch)
<p>Something else...?
</section>
<section>
<h3>Low VE from repeat vaccinations</h3>
<img src="images/mclean.jpg" style="width: 90%; height: 90%;">
<div class="citation">
<a href="https://www.ncbi.nlm.nih.gov/pubmed/25270645">
McLean et al. 2014
</a>
</div>
</section>
<section>
<h3>Eggs blamed in 2012-2013</h3>
<p>IVR-165 distinct from Vic/361 in ferrets, but not people
<img src="images/ivr165.png" style="width: 50%; height: 50%;">
<div class="citation">
<a href="http://www.biorxiv.org/content/early/2017/07/05/158550">
Cobey et al. 2017
</a>
</div>
</section>
</div>

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