This course is an introduction to RNA sequencing using Galaxy on Tufts University's Galaxy server. For support with this workshop or Galaxy, see our Galaxy documentation or email TTS Research Technology tts-research@tufts.edu
Bioinformatics for RNAseq has the following workflow steps
- Chrome web browser
- Account on Tufts High Performance Compute Cluster
- VPN if accessing the HPC from off campus
- Introduction Slides
- Introduction to Galaxy
- Next: Process Raw Reads
- Read Alignment
- Gene quantification
- Differential Expression
Thanks to bioinformatics intern Meg Farely for help developing this workshop. Much of this explanation has been adapted from these sources:

