CRISPRCAS

Repository containing python packages for running BE-DICT models. There are two packages:

• criscas for instantiating per-base model and
• haplotype for instantiating bystander model.

For more info about this research, see our preprint on biorxiv.

Installation

• git clone the repo and cd into it.
• Run pip install -e . to install the repo's python package.

Using Anaconda (optional) 🐍

The easiest way to install and manage Python packages on various OS platforms is through Anaconda. Once installed, any package (even if not available on Anaconda channel) could be installed using pip.

On Mac 

• Install Anaconda.

• git clone the repo and cd into it.

• Start a terminal and run

  # create an environment
  conda create --name bedict_crispr
  # activate the created environment (bedict_crispr)
  conda activate bedict_crispr
  # install anaconda
  conda install -c anaconda python=3.7
  # update all installed packages
  conda update -y --all
  # install pytorch 
  # Note cudatoolkit version depends on the version installed on your device
  # if there is no GPU run this command
  # conda install pytorch torchvision -c pytorch
  # see https://pytorch.org/
  conda install pytorch torchvision cudatoolkit=10.1 -c pytorch
  conda clean -ya
  # install criscas package (this package)
  pip install -e .

• After that we are ready to use the package with the trained BE-DICT models for base editing prediction.

BE-DICT model running demo 🏃‍♀️

We provide two notebooks that illustrate the use of BE-DICT (perbase) and (bystander) models under demo folder:

• BE-DICT perbase model
• BE-DICT bystander model

OS & Packages' version

The models were trained, tested and ran on Linux machine Ubuntu 18.04.3 LTS with one Tesla P4 GPU support. The version of the required packages used in setup.py were:

• numpy >= '1.18.1'
• scipy >= '1.4.1'
• pandas >= '1.0.1'
• scikit-learn >= '0.22.1'
• torch >= '1.7.1'
  • cudatoolkit=10.1
• matplotlib >= '3.1.3'
• seaborn >= '0.10.0'
• prettytable >= '2.0.0'
• tqdm >= '4.56.2'

Webapp 🕸️

A running instance supporting both models BE-DICT (perbase) & BE-DICT (bystander) can be accessed at this link.

Citation

If you find our work is useful in your research, please cite the following paper:

@article {Marquart2020.07.05.186544,
author = {Marquart, Kim F. and Allam, Ahmed and Janjuha, Sharan and Sintsova, Anna and Villiger, Lukas and Frey, Nina and Krauthammer, Michael and Schwank, Gerald},
title = {Predicting base editing outcomes with an attention-based deep learning algorithm trained on high-throughput target library screens},
elocation-id = {2020.07.05.186544},
year = {2020},
doi = {10.1101/2020.07.05.186544},
URL = {https://www.biorxiv.org/content/early/2020/07/05/2020.07.05.186544},
eprint = {https://www.biorxiv.org/content/early/2020/07/05/2020.07.05.186544.full.pdf},
journal = {bioRxiv}
}