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Vidjil – Analyzed human locus

The Vidjil web application is able to display multi-locus data, as long as this information is provided in the .vidjil file computed by the analysis program. Vidjil-algo currently analyzes the following locus, selecting the best locus for each read. The configuration of analyzed locus is done in the germline/homo-sapiens.g preset.

complete recombinationsincomplete/special recombinations
TRAVa-Ja
TRBVb-(Db)-JbTRB+Db-Jb
TRDVd-(Dd)-JdTRD+Vd-Dd3, Dd2-(Dd)-Jd, Dd2-Dd3
*TRA+D*Vd-(Dd)-Ja, Dd-Ja
TRGVg-Jg
IGHVh-(Dh)-JhIGH+Dh-Jh
IGLVl-Jl
IGKVk-JkIGK+Vk-KDE, INTRON-KDE
vidjil-algo option-g germline/homo-sapiens.g:TRA,TRB,TRD,TRG,IGH,IGL,IGK-g germline/homo-sapiens.g
server configurationmultimulti+inc

The detection of complete recombinations is reliable and should work provided that the reads are long enough (especially the J region).

The detection of incomplete/special recombinaisons is more challenging and may fail in some cases. In particular, as D genes may be very short, detecting TRD+ (Dd2/Dd3) and IGH+ (Dh-Jh) recombinations require to have reads with fairly conserved D genes or up/downstream regions.

Finally, the -2 command line option and the multi+inc+xxx server configuration try to detect unexpected or chimeric recombinations between genes of different germlines or on different strands (such as PCR dimers or +V/-V recombinations). These recombinations, tagged as xxx, can be technological artefacts or unusual biological recombinations.