diff --git a/packages/vite/rollup.config.ts b/packages/vite/rollup.config.ts
index 7a0a3305b6b794..716e75dbe2f48b 100644
--- a/packages/vite/rollup.config.ts
+++ b/packages/vite/rollup.config.ts
@@ -202,21 +202,6 @@ function createRuntimeConfig(isProduction: boolean) {
         isProduction ? false : './dist/node',
       ),
       esbuildMinifyPlugin({ minify: false, minifySyntax: true }),
-      {
-        name: 'replace bias',
-        transform(code, id) {
-          if (id.includes('@jridgewell+trace-mapping')) {
-            return {
-              code: code.replaceAll(
-                'bias === LEAST_UPPER_BOUND',
-                'true' +
-                  `/*${'bias === LEAST_UPPER_BOUND'.length - '/**/'.length - 'true'.length}*/`,
-              ),
-              map: null,
-            }
-          }
-        },
-      },
       bundleSizeLimit(45),
     ],
   })
diff --git a/packages/vite/src/node/ssr/runtime/__tests__/fixtures/has-error-deep.ts b/packages/vite/src/node/ssr/runtime/__tests__/fixtures/has-error-deep.ts
new file mode 100644
index 00000000000000..8da094a3fa4800
--- /dev/null
+++ b/packages/vite/src/node/ssr/runtime/__tests__/fixtures/has-error-deep.ts
@@ -0,0 +1,7 @@
+function crash(message: string) {
+  throw new Error(message)
+}
+
+export function main(): void {
+  crash('crash')
+}
diff --git a/packages/vite/src/node/ssr/runtime/__tests__/server-source-maps.spec.ts b/packages/vite/src/node/ssr/runtime/__tests__/server-source-maps.spec.ts
index c482a0464827f4..fd8973235af0b6 100644
--- a/packages/vite/src/node/ssr/runtime/__tests__/server-source-maps.spec.ts
+++ b/packages/vite/src/node/ssr/runtime/__tests__/server-source-maps.spec.ts
@@ -21,6 +21,11 @@ describe('vite-runtime initialization', async () => {
   const serializeStack = (runtime: ViteRuntime, err: Error) => {
     return err.stack!.split('\n')[1].replace(runtime.options.root, '<root>')
   }
+  const serializeStackDeep = (runtime: ViteRuntime, err: Error) => {
+    return err
+      .stack!.split('\n')
+      .map((s) => s.replace(runtime.options.root, '<root>'))
+  }
 
   it('source maps are correctly applied to stack traces', async ({
     runtime,
@@ -59,4 +64,16 @@ describe('vite-runtime initialization', async () => {
       '    at Module.throwError (<root>/fixtures/throws-error-method.ts:11:9)',
     )
   })
+
+  it('deep stacktrace', async ({ runtime }) => {
+    const methodError = await getError(async () => {
+      const mod = await runtime.executeUrl('/fixtures/has-error-deep.ts')
+      mod.main()
+    })
+    expect(serializeStackDeep(runtime, methodError).slice(0, 3)).toEqual([
+      'Error: crash',
+      '    at crash (<root>/fixtures/has-error-deep.ts:2:9)',
+      '    at Module.main (<root>/fixtures/has-error-deep.ts:6:3)',
+    ])
+  })
 })
diff --git a/playground/ssr-html/__tests__/ssr-html.spec.ts b/playground/ssr-html/__tests__/ssr-html.spec.ts
index fa9d12c7f6da41..92a2713420f2c3 100644
--- a/playground/ssr-html/__tests__/ssr-html.spec.ts
+++ b/playground/ssr-html/__tests__/ssr-html.spec.ts
@@ -98,6 +98,12 @@ describe.runIf(isServe)('stacktrace', () => {
       })
     }
   }
+
+  test('with Vite runtime', async () => {
+    await execFileAsync('node', ['test-stacktrace-runtime.js'], {
+      cwd: fileURLToPath(new URL('..', import.meta.url)),
+    })
+  })
 })
 
 describe.runIf(isServe)('network-imports', () => {
diff --git a/playground/ssr-html/src/has-error-deep.ts b/playground/ssr-html/src/has-error-deep.ts
new file mode 100644
index 00000000000000..8da094a3fa4800
--- /dev/null
+++ b/playground/ssr-html/src/has-error-deep.ts
@@ -0,0 +1,7 @@
+function crash(message: string) {
+  throw new Error(message)
+}
+
+export function main(): void {
+  crash('crash')
+}
diff --git a/playground/ssr-html/test-stacktrace-runtime.js b/playground/ssr-html/test-stacktrace-runtime.js
new file mode 100644
index 00000000000000..c2b8f670b5a089
--- /dev/null
+++ b/playground/ssr-html/test-stacktrace-runtime.js
@@ -0,0 +1,29 @@
+import { fileURLToPath } from 'node:url'
+import assert from 'node:assert'
+import { createServer, createViteRuntime } from 'vite'
+
+// same test case as packages/vite/src/node/ssr/runtime/__tests__/server-source-maps.spec.ts
+// implemented for e2e to catch build specific behavior
+
+const server = await createServer({
+  configFile: false,
+  root: fileURLToPath(new URL('.', import.meta.url)),
+  server: {
+    middlewareMode: true,
+  },
+})
+
+const runtime = await createViteRuntime(server, {
+  sourcemapInterceptor: 'prepareStackTrace',
+})
+
+const mod = await runtime.executeEntrypoint('/src/has-error-deep.ts')
+let error
+try {
+  mod.main()
+} catch (e) {
+  error = e
+} finally {
+  await server.close()
+}
+assert.match(error?.stack, /has-error-deep.ts:6:3/)