Permalink
Showing
with
5 additions
and
5 deletions.
-
+4
−4
README.md
-
+1
−1
src/scythe.c
|
|
@@ -1,4 +1,4 @@ |
|
|
-# Scythe - A Bayesian adapter trimmer (version 0.993 BETA)
|
|
|
+# Scythe - A Bayesian adapter trimmer (version 0.994 BETA)
|
|
|
|
|
|
Scythe and all supporting documentation
|
|
|
Copyright (c) Vince Buffalo, 2011-2014
|
|
|
@@ -69,18 +69,18 @@ If you'd like to use standard out, it is recommended you use the |
|
|
|
|
|
Also, more detailed output about matches can be obtained with:
|
|
|
|
|
|
- scythe -a adapter_file.fasta -o trimmed_sequences.fasta -m matches.txt sequences.fastq
|
|
|
+ scythe -a adapter_file.fasta -o trimmed_sequences.fastq -m matches.txt sequences.fastq
|
|
|
|
|
|
By default, Illumina's quality scheme (pipeline > 1.3) is used. Sanger
|
|
|
or Solexa (pipeline < 1.3) qualities can be specified with `-q`:
|
|
|
|
|
|
- scythe -a adapter_file.fasta -q solexa -o trimmed_sequences.fasta sequences.fastq
|
|
|
+ scythe -a adapter_file.fasta -q solexa -o trimmed_sequences.fastq sequences.fastq
|
|
|
|
|
|
Lastly, one can specify the minimum match length argument with `-n
|
|
|
<integer>` and the minimum length of sequence (discarded less than or
|
|
|
equal to this parameter) to keep after trimming with `-M <integer>`:
|
|
|
|
|
|
- scythe -a adapter_file.fasta -n 0 -M 10 -o trimmed_sequences.fasta sequences.fastq
|
|
|
+ scythe -a adapter_file.fasta -n 0 -M 10 -o trimmed_sequences.fastq sequences.fastq
|
|
|
|
|
|
The default is 5. If this pre-processing is upstream of assembly on a
|
|
|
very contaminated lane, decreasing this parameter could lead to *very*
|
|
|
|
|
|
@@ -30,7 +30,7 @@ static const float default_prior = 0.3; |
|
|
#endif
|
|
|
|
|
|
#ifndef VERSION
|
|
|
-#define VERSION 0.993
|
|
|
+#define VERSION 0.994
|
|
|
#endif
|
|
|
|
|
|
/* Options drawn from GNU's coreutils/src/system.h */
|
|
|
|
0 comments on commit
20d3cff