diff --git a/.changeset/khaki-hairs-end.md b/.changeset/khaki-hairs-end.md
new file mode 100644
index 0000000000..377b40ec38
--- /dev/null
+++ b/.changeset/khaki-hairs-end.md
@@ -0,0 +1,5 @@
+---
+'@astrojs/starlight': patch
+---
+
+Fixes a UI strings translation issue for sites configured with a single non-root language different from English.
diff --git a/packages/starlight/__tests__/i18n-non-root-single-locale/config.test.ts b/packages/starlight/__tests__/i18n-non-root-single-locale/config.test.ts
new file mode 100644
index 0000000000..a7b7262eb3
--- /dev/null
+++ b/packages/starlight/__tests__/i18n-non-root-single-locale/config.test.ts
@@ -0,0 +1,17 @@
+import config from 'virtual:starlight/user-config';
+import { expect, test } from 'vitest';
+
+test('test suite is using correct env', () => {
+	expect(config.title).toBe('i18n with a non-root single locale');
+});
+
+test('config.isMultilingual is false with a single locale', () => {
+	expect(config.isMultilingual).toBe(false);
+	expect(config.locales).keys('fr');
+});
+
+test('config.defaultLocale is populated from default locale', () => {
+	expect(config.defaultLocale.lang).toBe('fr');
+	expect(config.defaultLocale.dir).toBe('ltr');
+	expect(config.defaultLocale.locale).toBe('fr');
+});
diff --git a/packages/starlight/__tests__/i18n-non-root-single-locale/localizedUrl.test.ts b/packages/starlight/__tests__/i18n-non-root-single-locale/localizedUrl.test.ts
new file mode 100644
index 0000000000..49ef69aa7f
--- /dev/null
+++ b/packages/starlight/__tests__/i18n-non-root-single-locale/localizedUrl.test.ts
@@ -0,0 +1,26 @@
+import { describe, expect, test } from 'vitest';
+import { localizedUrl } from '../../utils/localizedUrl';
+
+describe('with `build.output: "directory"`', () => {
+	test('it has no effect in a monolingual project with a non-root single locale', () => {
+		const url = new URL('https://example.com/fr/guide/');
+		expect(localizedUrl(url, 'fr').href).toBe(url.href);
+	});
+
+	test('has no effect on index route in a monolingual project with a non-root single locale', () => {
+		const url = new URL('https://example.com/fr/');
+		expect(localizedUrl(url, 'fr').href).toBe(url.href);
+	});
+});
+
+describe('with `build.output: "file"`', () => {
+	test('it has no effect in a monolingual project with a non-root single locale', () => {
+		const url = new URL('https://example.com/fr/guide.html');
+		expect(localizedUrl(url, 'fr').href).toBe(url.href);
+	});
+
+	test('has no effect on index route in a monolingual project with a non-root single locale', () => {
+		const url = new URL('https://example.com/fr.html');
+		expect(localizedUrl(url, 'fr').href).toBe(url.href);
+	});
+});
diff --git a/packages/starlight/__tests__/i18n-non-root-single-locale/route-data.test.ts b/packages/starlight/__tests__/i18n-non-root-single-locale/route-data.test.ts
new file mode 100644
index 0000000000..dbf5d35481
--- /dev/null
+++ b/packages/starlight/__tests__/i18n-non-root-single-locale/route-data.test.ts
@@ -0,0 +1,19 @@
+import { expect, test, vi } from 'vitest';
+import { generateRouteData } from '../../utils/route-data';
+import { routes } from '../../utils/routing';
+
+vi.mock('astro:content', async () =>
+	(await import('../test-utils')).mockedAstroContent({
+		docs: [['fr/index.mdx', { title: 'Accueil' }]],
+	})
+);
+
+test('includes localized labels (fr)', () => {
+	const route = routes[0]!;
+	const data = generateRouteData({
+		props: { ...route, headings: [{ depth: 1, slug: 'heading-1', text: 'Heading 1' }] },
+		url: new URL('https://example.com'),
+	});
+	expect(data.labels).toBeDefined();
+	expect(data.labels['skipLink.label']).toBe('Aller au contenu');
+});
diff --git a/packages/starlight/__tests__/i18n-non-root-single-locale/routing.test.ts b/packages/starlight/__tests__/i18n-non-root-single-locale/routing.test.ts
new file mode 100644
index 0000000000..2caef2f599
--- /dev/null
+++ b/packages/starlight/__tests__/i18n-non-root-single-locale/routing.test.ts
@@ -0,0 +1,35 @@
+import { expect, test, vi } from 'vitest';
+import { routes } from '../../utils/routing';
+
+vi.mock('astro:content', async () =>
+	(await import('../test-utils')).mockedAstroContent({
+		docs: [
+			['fr/index.mdx', { title: 'Accueil' }],
+			['en/index.mdx', { title: 'Home page' }],
+		],
+	})
+);
+
+test('route slugs are normalized', () => {
+	const indexRoute = routes.find((route) => route.id.startsWith('fr/index.md'));
+	expect(indexRoute?.slug).toBe('fr');
+});
+
+test('routes for the configured locale have locale data added', () => {
+	for (const route of routes) {
+		if (route.id.startsWith('fr')) {
+			expect(route.lang).toBe('fr');
+			expect(route.dir).toBe('ltr');
+			expect(route.locale).toBe('fr');
+		} else {
+			expect(route.lang).toBe('fr');
+			expect(route.dir).toBe('ltr');
+			expect(route.locale).toBeUndefined();
+		}
+	}
+});
+
+test('does not mark any route as fallback routes', () => {
+	const fallbacks = routes.filter((route) => route.isFallback);
+	expect(fallbacks.length).toBe(0);
+});
diff --git a/packages/starlight/__tests__/i18n-non-root-single-locale/slugs.test.ts b/packages/starlight/__tests__/i18n-non-root-single-locale/slugs.test.ts
new file mode 100644
index 0000000000..1904ee113b
--- /dev/null
+++ b/packages/starlight/__tests__/i18n-non-root-single-locale/slugs.test.ts
@@ -0,0 +1,37 @@
+import { describe, expect, test } from 'vitest';
+import { localeToLang, localizedId, localizedSlug, slugToLocaleData } from '../../utils/slugs';
+
+describe('slugToLocaleData', () => {
+	test('returns default "fr" locale', () => {
+		expect(slugToLocaleData('fr/test').locale).toBe('fr');
+		expect(slugToLocaleData('fr/dir/test').locale).toBe('fr');
+	});
+	test('returns default locale "fr" lang', () => {
+		expect(slugToLocaleData('fr/test').lang).toBe('fr');
+		expect(slugToLocaleData('fr/dir/test').lang).toBe('fr');
+	});
+	test('returns default locale "ltr" dir', () => {
+		expect(slugToLocaleData('fr/test').dir).toBe('ltr');
+		expect(slugToLocaleData('fr/dir/test').dir).toBe('ltr');
+	});
+});
+
+describe('localeToLang', () => {
+	test('returns lang for default locale', () => {
+		expect(localeToLang('fr')).toBe('fr');
+	});
+});
+
+describe('localizedId', () => {
+	test('returns unchanged for default locale', () => {
+		expect(localizedId('fr/test.md', 'fr')).toBe('fr/test.md');
+	});
+});
+
+describe('localizedSlug', () => {
+	test('returns unchanged for default locale', () => {
+		expect(localizedSlug('fr', 'fr')).toBe('fr');
+		expect(localizedSlug('fr/test', 'fr')).toBe('fr/test');
+		expect(localizedSlug('fr/dir/test', 'fr')).toBe('fr/dir/test');
+	});
+});
diff --git a/packages/starlight/__tests__/i18n-non-root-single-locale/src/content/i18n/fr.json b/packages/starlight/__tests__/i18n-non-root-single-locale/src/content/i18n/fr.json
new file mode 100644
index 0000000000..2b27eb1edf
--- /dev/null
+++ b/packages/starlight/__tests__/i18n-non-root-single-locale/src/content/i18n/fr.json
@@ -0,0 +1,3 @@
+{
+  "page.editLink": "Changer cette page"
+}
diff --git a/packages/starlight/__tests__/i18n-non-root-single-locale/translations-fs.test.ts b/packages/starlight/__tests__/i18n-non-root-single-locale/translations-fs.test.ts
new file mode 100644
index 0000000000..1248df1ebe
--- /dev/null
+++ b/packages/starlight/__tests__/i18n-non-root-single-locale/translations-fs.test.ts
@@ -0,0 +1,43 @@
+import { describe, expect, test } from 'vitest';
+import { createTranslationSystemFromFs } from '../../utils/translations-fs';
+
+describe('createTranslationSystemFromFs', () => {
+	test('creates a translation system that returns default strings', () => {
+		const useTranslations = createTranslationSystemFromFs(
+			{
+				locales: { fr: { label: 'Français', dir: 'ltr' } },
+				defaultLocale: { label: 'Français', locale: 'fr', dir: 'ltr' },
+			},
+			// Using non-existent `_src/` to ignore custom files in this test fixture.
+			{ srcDir: new URL('./_src/', import.meta.url) }
+		);
+		const t = useTranslations('fr');
+		expect(t('page.editLink')).toMatchInlineSnapshot('"Modifier cette page"');
+	});
+
+	test('creates a translation system that uses custom strings', () => {
+		const useTranslations = createTranslationSystemFromFs(
+			{
+				locales: { fr: { label: 'Français', dir: 'ltr' } },
+				defaultLocale: { label: 'Français', locale: 'fr', dir: 'ltr' },
+			},
+			// Using `src/` to load custom files in this test fixture.
+			{ srcDir: new URL('./src/', import.meta.url) }
+		);
+		const t = useTranslations('fr');
+		expect(t('page.editLink')).toMatchInlineSnapshot('"Changer cette page"');
+	});
+
+	test('returns translation for unknown language', () => {
+		const useTranslations = createTranslationSystemFromFs(
+			{
+				locales: { fr: { label: 'Français', dir: 'ltr', lang: 'fr' } },
+				defaultLocale: { label: 'Français', locale: 'fr', dir: 'ltr' },
+			},
+			// Using `src/` to load custom files in this test fixture.
+			{ srcDir: new URL('./src/', import.meta.url) }
+		);
+		const t = useTranslations('ar');
+		expect(t('page.editLink')).toMatchInlineSnapshot('"Changer cette page"');
+	});
+});
diff --git a/packages/starlight/__tests__/i18n-non-root-single-locale/translations-with-user-config.test.ts b/packages/starlight/__tests__/i18n-non-root-single-locale/translations-with-user-config.test.ts
new file mode 100644
index 0000000000..bc76cc2860
--- /dev/null
+++ b/packages/starlight/__tests__/i18n-non-root-single-locale/translations-with-user-config.test.ts
@@ -0,0 +1,17 @@
+import { describe, expect, test, vi } from 'vitest';
+import translations from '../../translations';
+import { useTranslations } from '../../utils/translations';
+
+vi.mock('astro:content', async () =>
+	(await import('../test-utils')).mockedAstroContent({
+		i18n: [['fr', { 'page.editLink': 'Modifier cette doc!' }]],
+	})
+);
+
+describe('useTranslations()', () => {
+	test('uses user-defined translations', () => {
+		const t = useTranslations('fr');
+		expect(t('page.editLink')).toBe('Modifier cette doc!');
+		expect(t('page.editLink')).not.toBe(translations.fr?.['page.editLink']);
+	});
+});
diff --git a/packages/starlight/__tests__/i18n-non-root-single-locale/translations.test.ts b/packages/starlight/__tests__/i18n-non-root-single-locale/translations.test.ts
new file mode 100644
index 0000000000..3287cdf27a
--- /dev/null
+++ b/packages/starlight/__tests__/i18n-non-root-single-locale/translations.test.ts
@@ -0,0 +1,24 @@
+import { describe, expect, test } from 'vitest';
+import translations from '../../translations';
+import { useTranslations } from '../../utils/translations';
+
+describe('built-in translations', () => {
+	test('includes French', () => {
+		expect(translations).toHaveProperty('fr');
+	});
+});
+
+describe('useTranslations()', () => {
+	test('works when no i18n collection is available', () => {
+		const t = useTranslations('fr');
+		expect(t).toBeTypeOf('function');
+		expect(t('page.editLink')).toBe(translations.fr?.['page.editLink']);
+	});
+
+	test('returns default locale for unknown language', () => {
+		const locale = 'xx';
+		expect(translations).not.toHaveProperty(locale);
+		const t = useTranslations(locale);
+		expect(t('page.editLink')).toBe(translations.fr?.['page.editLink']);
+	});
+});
diff --git a/packages/starlight/__tests__/i18n-non-root-single-locale/vitest.config.ts b/packages/starlight/__tests__/i18n-non-root-single-locale/vitest.config.ts
new file mode 100644
index 0000000000..be081a7c57
--- /dev/null
+++ b/packages/starlight/__tests__/i18n-non-root-single-locale/vitest.config.ts
@@ -0,0 +1,9 @@
+import { defineVitestConfig } from '../test-config';
+
+export default defineVitestConfig({
+	title: 'i18n with a non-root single locale',
+	defaultLocale: 'fr',
+	locales: {
+		fr: { label: 'Français', lang: 'fr' },
+	},
+});
diff --git a/packages/starlight/utils/user-config.ts b/packages/starlight/utils/user-config.ts
index f77f4e9058..8e566346b4 100644
--- a/packages/starlight/utils/user-config.ts
+++ b/packages/starlight/utils/user-config.ts
@@ -212,16 +212,20 @@ const UserConfigSchema = z.object({
 
 export const StarlightConfigSchema = UserConfigSchema.strict().transform(
 	({ locales, defaultLocale, ...config }, ctx) => {
-		if (locales !== undefined && Object.keys(locales).length > 1) {
-			// This is a multilingual site (more than one locale configured).
+		const configuredLocales = Object.keys(locales ?? {});
+
+		// This is a multilingual site (more than one locale configured) or a monolingual site with
+		// only one locale configured (not a root locale).
+		// Monolingual sites with only one non-root locale needs their configuration to be defined in
+		// `config.locales` so that slugs can be correctly generated by taking into consideration the
+		// base path at which a language is served which is the key of the `config.locales` object.
+		if (locales !== undefined && configuredLocales.length >= 1) {
 			// Make sure we can find the default locale and if not, help the user set it.
 			// We treat the root locale as the default if present and no explicit default is set.
 			const defaultLocaleConfig = locales[defaultLocale || 'root'];
 
 			if (!defaultLocaleConfig) {
-				const availableLocales = Object.keys(locales)
-					.map((l) => `"${l}"`)
-					.join(', ');
+				const availableLocales = configuredLocales.map((l) => `"${l}"`).join(', ');
 				ctx.addIssue({
 					code: 'custom',
 					message:
@@ -235,14 +239,15 @@ export const StarlightConfigSchema = UserConfigSchema.strict().transform(
 			return {
 				...config,
 				/** Flag indicating if this site has multiple locales set up. */
-				isMultilingual: true,
+				isMultilingual: configuredLocales.length > 1,
 				/** Full locale object for this site’s default language. */
 				defaultLocale: { ...defaultLocaleConfig, locale: defaultLocale },
 				locales,
 			} as const;
 		}
 
-		// This is a monolingual site, so things are pretty simple.
+		// This is a monolingual site with no locales configured or only a root locale, so things are
+		// pretty simple.
 		return {
 			...config,
 			/** Flag indicating if this site has multiple locales set up. */