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Matlab-Single-Molecule

Scripts to analyze single molecule fluorescence experiments. Derived from work done in Joseph D. Puglisi's lab at Stanford University and Peter Walter's lab at the University of California San Francisco.

-fretSelector function selects cleans up large datasets by selecting traces that show temporal anti-correlation between donor and acceptor fluorophore channels, and are therefore likely to have fluorescence resonace energy transfer (FRET) signal.

-stateAssign function allows user to make state assignments for single molecule fluorescent observations over multiple color channels and multiple assignment classes. A screencast is available at: http://youtu.be/uD7A-FJNoH0

-simulator script is an example of a stochastic simulation algorithm that will simulate signal recognition-ribosome binding experiments under single molecule experiments.

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Scripts to analyze single molecule fluorescence experiments

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