diff --git a/docs/changes.txt b/docs/changes.txt
index 2c83bf6b0f3e..648c9b69b084 100644
--- a/docs/changes.txt
+++ b/docs/changes.txt
@@ -243,6 +243,7 @@ All (GUI):
 - Fix AUI floating pane position when dragging (Konstantin S. Matveyev, #22533).
 - Add wxStyledTextCtrl support to XRC schema file.
 - Fix script parsing in wxLocaleIdent (Ulrich Telle).
+- Make generic wxListCtrl separator lines more visible in dark themes (#22664).
 
 wxGTK:
 
diff --git a/include/wx/generic/private/listctrl.h b/include/wx/generic/private/listctrl.h
index 62e66edea0f8..fa2e725aff02 100644
--- a/include/wx/generic/private/listctrl.h
+++ b/include/wx/generic/private/listctrl.h
@@ -893,7 +893,7 @@ class wxListMainWindow : public wxWindow
     // get the colour to be used for drawing the rules
     wxColour GetRuleColour() const
     {
-        return wxSystemSettings::GetColour(wxSYS_COLOUR_3DLIGHT);
+        return wxSystemSettings::GetColour(wxSYS_COLOUR_GRAYTEXT);
     }
 
 private: