diff --git a/tests/drivers/clock_control/clock_control_api/boards/sam_e54_xpro.conf b/tests/drivers/clock_control/clock_control_api/boards/sam_e54_xpro.conf
new file mode 100644
index 0000000000000..772fd036e4934
--- /dev/null
+++ b/tests/drivers/clock_control/clock_control_api/boards/sam_e54_xpro.conf
@@ -0,0 +1,4 @@
+# Copyright (c) 2025 Microchip Technology Inc.
+# SPDX-License-Identifier: Apache-2.0
+
+CONFIG_CLOCK_CONTROL_MCHP_ASYNC_ON=y
diff --git a/tests/drivers/clock_control/clock_control_api/boards/sam_e54_xpro.overlay b/tests/drivers/clock_control/clock_control_api/boards/sam_e54_xpro.overlay
new file mode 100644
index 0000000000000..82d45b0a92885
--- /dev/null
+++ b/tests/drivers/clock_control/clock_control_api/boards/sam_e54_xpro.overlay
@@ -0,0 +1,28 @@
+/*
+ * Copyright (c) 2025 Microchip Technology Inc.
+ *
+ * SPDX-License-Identifier: Apache-2.0
+ */
+
+&clock {
+	mclkperiph: mclkperiph {
+		compatible = "microchip,sam-d5x-e5x-mclkperiph";
+		#clock-cells = <1>;
+
+		sercom3 {
+			subsystem = <CLOCK_MCHP_MCLKPERIPH_ID_APBB_SERCOM3>;
+			mclk-en = <1>;
+		};
+	};
+
+	gclkperiph: gclkperiph {
+		compatible = "microchip,sam-d5x-e5x-gclkperiph";
+		#clock-cells = <1>;
+
+		sercom4 {
+			subsystem = <CLOCK_MCHP_GCLKPERIPH_ID_SERCOM4_CORE>;
+			gclkperiph-src = "gclk0";
+			gclkperiph-en = <1>;
+		};
+	};
+};
diff --git a/tests/drivers/clock_control/clock_control_api/src/mchp_device_subsys.h b/tests/drivers/clock_control/clock_control_api/src/mchp_device_subsys.h
new file mode 100644
index 0000000000000..11a4377eebe80
--- /dev/null
+++ b/tests/drivers/clock_control/clock_control_api/src/mchp_device_subsys.h
@@ -0,0 +1,24 @@
+/*
+ * Copyright (c) 2025 Microchip Technology Inc.
+ *
+ * SPDX-License-Identifier: Apache-2.0
+ */
+
+#include "device_subsys.h"
+#include <zephyr/drivers/clock_control/mchp_clock_sam_d5x_e5x.h>
+#include <zephyr/dt-bindings/clock/mchp_sam_d5x_e5x_clock.h>
+
+#define XOSC_STARTUP_US 500
+
+static const struct device_subsys_data subsys_data[] = {
+	{.subsys = (void *)CLOCK_MCHP_MCLKPERIPH_ID_APBB_SERCOM3},
+	{.subsys = (void *)CLOCK_MCHP_GCLKPERIPH_ID_SERCOM4_CORE},
+	{
+		.subsys = (void *)CLOCK_MCHP_XOSC_ID_XOSC1,
+		.startup_us = XOSC_STARTUP_US,
+	},
+	{.subsys = (void *)CLOCK_MCHP_XOSC32K_ID_XOSC32K}};
+
+static const struct device_data devices[] = {{.dev = DEVICE_DT_GET(DT_NODELABEL(clock)),
+					      .subsys_data = subsys_data,
+					      .subsys_cnt = ARRAY_SIZE(subsys_data)}};
diff --git a/tests/drivers/clock_control/clock_control_api/src/test_clock_control.c b/tests/drivers/clock_control/clock_control_api/src/test_clock_control.c
index 91af56bd3d29f..e1c0eb2a2130c 100644
--- a/tests/drivers/clock_control/clock_control_api/src/test_clock_control.c
+++ b/tests/drivers/clock_control/clock_control_api/src/test_clock_control.c
@@ -14,6 +14,8 @@ LOG_MODULE_REGISTER(test);
 #include "esp32_device_subsys.h"
 #elif DT_HAS_COMPAT_STATUS_OKAY(silabs_series_clock)
 #include "silabs_device_subsys.h"
+#elif DT_HAS_COMPAT_STATUS_OKAY(microchip_sam_d5x_e5x_clock)
+#include "mchp_device_subsys.h"
 #else
 #error "Unsupported board"
 #endif
diff --git a/tests/drivers/clock_control/clock_control_api/testcase.yaml b/tests/drivers/clock_control/clock_control_api/testcase.yaml
index d3c2669641fc2..4e3d86d831863 100644
--- a/tests/drivers/clock_control/clock_control_api/testcase.yaml
+++ b/tests/drivers/clock_control/clock_control_api/testcase.yaml
@@ -14,6 +14,7 @@ tests:
       - xg27_dk2602a
       - xg29_rb4412a
       - bg29_rb4420a
+      - sam_e54_xpro
     integration_platforms:
       - esp32_devkitc/esp32/procpu
   drivers.clock.clock_control_nrf5: