diff --git a/subsys/net/l2/openthread/openthread_border_router.c b/subsys/net/l2/openthread/openthread_border_router.c
index 2576f468d141c..417507b03ccff 100644
--- a/subsys/net/l2/openthread/openthread_border_router.c
+++ b/subsys/net/l2/openthread/openthread_border_router.c
@@ -206,8 +206,6 @@ void openthread_set_bbr_multicast_listener_cb(openthread_bbr_multicast_listener_
 					      void *context)
 {
 	__ASSERT(cb != NULL, "Receive callback is not set");
-	__ASSERT(openthread_instance != NULL, "OpenThread instance is not "
-					      "initialized");
 
 	openthread_mutex_lock();
 	otBackboneRouterSetMulticastListenerCallback(openthread_get_default_instance(), cb,
diff --git a/tests/net/all/testcase.yaml b/tests/net/all/testcase.yaml
index fc03be2510507..bf0a2ba2a8451 100644
--- a/tests/net/all/testcase.yaml
+++ b/tests/net/all/testcase.yaml
@@ -22,3 +22,93 @@ tests:
   net.build.compat_namespacing:
     extra_configs:
       - CONFIG_NET_NAMESPACE_COMPAT_MODE=y
+  # Compile test OpenThread
+  net.build.no_compat_namespacing_openthread:
+    build_only: true
+    tags:
+      - net
+      - openthread
+    depends_on: openthread
+    platform_allow:
+      - frdm_rw612
+    extra_configs:
+      - CONFIG_NET_NAMESPACE_COMPAT_MODE=n
+      - CONFIG_POSIX_API=n
+      - CONFIG_SHELL_GETOPT=n
+      - CONFIG_NET_L2_WIFI_SHELL=n
+      - CONFIG_NET_L2_OPENTHREAD=y
+      - CONFIG_CPP=y
+      - CONFIG_NET_SLIP_TAP=n
+      - CONFIG_NET_QEMU_SLIP=n
+      - CONFIG_LWM2M_RESOURCE_DATA_CACHE_SUPPORT=n
+      - CONFIG_LWM2M_RW_JSON_SUPPORT=n
+      - CONFIG_USERSPACE=y
+      - CONFIG_NXP_MONOLITHIC_NBU=n
+      - CONFIG_NXP_MONOLITHIC_WIFI=n
+      - CONFIG_NVS=y
+      - CONFIG_NXP_RF_IMU=y
+      - CONFIG_NXP_FW_LOADER=y
+      - CONFIG_HDLC_RCP_IF=y
+      - CONFIG_OPENTHREAD_MANUAL_START=y
+      - CONFIG_OPENTHREAD_ZEPHYR_BORDER_ROUTER=y
+      - CONFIG_OPENTHREAD_RCP_RESTORATION_MAX_COUNT=10
+      - CONFIG_OPENTHREAD_ZEPHYR_BORDER_ROUTER_VENDOR_NAME="Zephyr"
+      - CONFIG_OPENTHREAD_ZEPHYR_BORDER_ROUTER_BASE_SERVICE_NAME="Zephyr-BorderRouter"
+      - CONFIG_OPENTHREAD_BACKBONE_ROUTER_MULTICAST_ROUTING=y
+      - CONFIG_OPENTHREAD_SLAAC=y
+      - CONFIG_OPENTHREAD_ENABLE_SERVICE=y
+      - CONFIG_OPENTHREAD_BORDER_ROUTER=y
+      - CONFIG_OPENTHREAD_BORDER_ROUTING=y
+      - CONFIG_OPENTHREAD_BORDER_ROUTING_COUNTERS=y
+      - CONFIG_OPENTHREAD_BORDER_AGENT=y
+      - CONFIG_OPENTHREAD_BORDER_AGENT_ID=y
+      - CONFIG_OPENTHREAD_NETDATA_PUBLISHER=y
+      - CONFIG_OPENTHREAD_BACKBONE_ROUTER=y
+      - CONFIG_OPENTHREAD_NAT64_TRANSLATOR=y
+      - CONFIG_OPENTHREAD_NAT64_BORDER_ROUTING=y
+      - CONFIG_OPENTHREAD_DNSSD_SERVER=y
+      - CONFIG_OPENTHREAD_EXTERNAL_HEAP=y
+      - CONFIG_OPENTHREAD_JOINER=y
+      - CONFIG_OPENTHREAD_COMMISSIONER=y
+      - CONFIG_OPENTHREAD_PLATFORM_UDP=y
+      - CONFIG_OPENTHREAD_MULTICAST_DNS=y
+      - CONFIG_OPENTHREAD_MAX_STATECHANGE_HANDLERS=3
+      - CONFIG_OPENTHREAD_INTERFACE_EARLY_UP=y
+      - CONFIG_OPENTHREAD_ECDSA=y
+      - CONFIG_OPENTHREAD_IP6_FRAGM=y
+      - CONFIG_OPENTHREAD_COAP=y
+      - CONFIG_OPENTHREAD_SETTINGS_RAM=y
+      - CONFIG_OPENTHREAD_BORDER_AGENT_EPHEMERAL_KEY_ENABLE=y
+      - CONFIG_OPENTHREAD_VERHOEFF_CHECKSUM=y
+      - CONFIG_OPENTHREAD_BORDER_AGENT_MESHCOP_SERVICE=y
+      - CONFIG_OPENTHREAD_TREL=y
+      - CONFIG_OPENTHREAD_TREL_MANAGE_DNSSD=y
+      - CONFIG_OPENTHREAD_BORDER_ROUTING_DHCP6_PD=y
+      - CONFIG_OPENTHREAD_BORDER_ROUTING_DHCP6_PD_CLIENT=y
+      - CONFIG_OPENTHREAD_DHCP6_CLIENT=y
+      - CONFIG_OPENTHREAD_DHCP6_SERVER=y
+      - CONFIG_OPENTHREAD_DNS_UPSTREAM_QUERY=y
+      - CONFIG_OPENTHREAD_SRP_CLIENT=y
+      - CONFIG_OPENTHREAD_SRP_SERVER=y
+      - CONFIG_OPENTHREAD_SRP_ADV_PROXY=y
+      - CONFIG_OPENTHREAD_DNSSD_DISCOVERY_PROXY=y
+      - CONFIG_OPENTHREAD_PING_SENDER=y
+      - CONFIG_OPENTHREAD_REFERENCE_DEVICE=y
+      - CONFIG_OPENTHREAD_LINK_METRICS_INITIATOR=y
+      - CONFIG_OPENTHREAD_DUA=y
+      - CONFIG_OPENTHREAD_MLR=y
+      - CONFIG_OPENTHREAD_DNS_CLIENT=y
+      - CONFIG_OPENTHREAD_DNS_CLIENT_OVER_TCP=y
+      - CONFIG_OPENTHREAD_TCP_ENABLE=y
+      - CONFIG_OPENTHREAD_NETDIAG_VENDOR_INFO=y
+      - CONFIG_OPENTHREAD_LINK_METRICS_SUBJECT=y
+      - CONFIG_OPENTHREAD_LINK_METRICS_MANAGER=y
+      - CONFIG_OPENTHREAD_LINK_METRICS_INITIATOR=y
+      - CONFIG_OPENTHREAD_MAC_FILTER=y
+      - CONFIG_NET_SOCKETS_INET_RAW=y
+      - CONFIG_OPENTHREAD_THREAD_VERSION_1_4=y
+      - CONFIG_NET_IPV6_PE=n
+      - CONFIG_NET_L2_IEEE802154=n
+      - CONFIG_NET_ROUTE_MCAST=y
+      - CONFIG_OPENTHREAD_SHELL=y
+      - CONFIG_DNS_RESOLVER_PACKET_FORWARDING=y