fix(shared-data): Glycerol 96ch max flowrate should be 228 not 250 #18856
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## chore_release-8.5.0 #18856 +/- ##
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- Coverage 25.70% 24.43% -1.28%
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Files 3265 3258 -7
Lines 282652 281757 -895
Branches 29924 29872 -52
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- Hits 72664 68844 -3820
- Misses 209966 212886 +2920
- Partials 22 27 +5
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andySigler
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@andySigler @ncdiehl Please someone verify if that's an okay way to check this It looks like your numbers disagreed import json
from opentrons import protocol_api, types
metadata = {
"created": "2025-07-08T20:51:12.902Z",
"lastModified": "2025-07-08T21:10:18.918Z",
"protocolDesigner": "8.5.0-alpha.2",
"source": "Protocol Designer",
}
requirements = {"robotType": "Flex", "apiLevel": "2.24"}
def run(protocol: protocol_api.ProtocolContext) -> None:
# Load Adapters:
adapter_1 = protocol.load_adapter(
"opentrons_flex_96_tiprack_adapter",
location="C2",
namespace="opentrons",
)
# Load Labware:
tip_rack_1 = adapter_1.load_labware(
"opentrons_flex_96_tiprack_1000ul",
namespace="opentrons",
)
reservoir_1 = protocol.load_labware(
"agilent_1_reservoir_290ml",
location="B1",
namespace="opentrons",
)
# Load Pipettes:
pipette = protocol.load_instrument("flex_96channel_1000", tip_racks=[tip_rack_1])
# Load Trash Bins:
trash_bin_1 = protocol.load_trash_bin("A3")
# Define Liquids:
liquid_1 = protocol.define_liquid(
"f",
display_color="#b925ff",
)
# Load Liquids:
reservoir_1.load_liquid(
wells=["A1"],
liquid=liquid_1,
volume=124124,
)
# Load Liquid Classes:
glycerol_50_base_class = protocol.get_liquid_class("glycerol_50")
# PROTOCOL STEPS
# Step 1:
pipette.configure_nozzle_layout(
protocol_api.ALL,
tip_racks=[tip_rack_1],
)
pipette.transfer_with_liquid_class(
volume=1000,
source=[reservoir_1["A1"]],
dest=[reservoir_1["A1"]],
new_tip="always",
trash_location=trash_bin_1,
group_wells=False,
keep_last_tip=True,
liquid_class=protocol.define_liquid_class(
name="transfer_step_1",
base_liquid_class=glycerol_50_base_class,
properties={"flex_96channel_1000": {"opentrons/opentrons_flex_96_tiprack_1000ul/1": {
"aspirate": {
"aspirate_position": {
"offset": {"x": 0, "y": 0, "z": 2},
"position_reference": "well-bottom",
},
"flow_rate_by_volume": [(0, 200)],
"pre_wet": False,
"correction_by_volume": [(0, 12)],
"delay": {"enabled": True, "duration": 0.7},
"mix": {"enabled": False},
"submerge": {
"delay": {"enabled": False},
"speed": 10,
"start_position": {
"offset": {"x": 0, "y": 0, "z": 2},
"position_reference": "well-top",
},
},
"retract": {
"air_gap_by_volume": [(0, 0)],
"delay": {"enabled": False},
"end_position": {
"offset": {"x": 0, "y": 0, "z": 2},
"position_reference": "well-top",
},
"speed": 10,
"touch_tip": {"enabled": False},
},
},
"dispense": {
"dispense_position": {
"offset": {"x": 0, "y": 0, "z": 2},
"position_reference": "well-bottom",
},
"push_out_by_volume": [(0, 35)],
"flow_rate_by_volume": [(0, 223)],
"correction_by_volume": [(0, 12)],
"delay": {"enabled": True, "duration": 0.5},
"mix": {"enabled": False},
"submerge": {
"delay": {"enabled": False},
"speed": 10,
"start_position": {
"offset": {"x": 0, "y": 0, "z": 2},
"position_reference": "well-top",
},
},
"retract": {
"air_gap_by_volume": [(0, 0)],
"delay": {"enabled": False},
"end_position": {
"offset": {"x": 0, "y": 0, "z": 2},
"position_reference": "well-top",
},
"speed": 10,
"touch_tip": {"enabled": False},
"blowout": {"enabled": False},
},
},
}}},
),
)
pipette.drop_tip()
DESIGNER_APPLICATION = """{"robot":{"model":"OT-3 Standard"},"designerApplication":{"name":"opentrons/protocol-designer","version":"8.5.0","data":{"pipetteTiprackAssignments":{"77256c19-9f4f-4229-853e-3e2c6e1ce7d0":["opentrons/opentrons_flex_96_tiprack_1000ul/1"]},"dismissedWarnings":{"form":[],"timeline":[]},"ingredients":{"0":{"displayName":"f","displayColor":"#b925ff","liquidClass":"glycerol50V1","description":null,"liquidGroupId":"0"}},"ingredLocations":{"1ce7c748-a641-4285-811b-639ee5ad4b81:opentrons/agilent_1_reservoir_290ml/3":{"A1":{"0":{"volume":124124}}}},"savedStepForms":{"__INITIAL_DECK_SETUP_STEP__":{"stepType":"manualIntervention","id":"__INITIAL_DECK_SETUP_STEP__","labwareLocationUpdate":{"5acddb58-a2b4-4d20-b9f7-fe6eeaeef5aa:opentrons/opentrons_flex_96_tiprack_adapter/1":"C2","3996b0b6-c6ad-4f3a-91e5-b680f37ee9ea:opentrons/opentrons_flex_96_tiprack_1000ul/1":"5acddb58-a2b4-4d20-b9f7-fe6eeaeef5aa:opentrons/opentrons_flex_96_tiprack_adapter/1","1ce7c748-a641-4285-811b-639ee5ad4b81:opentrons/agilent_1_reservoir_290ml/3":"B1"},"pipetteLocationUpdate":{"77256c19-9f4f-4229-853e-3e2c6e1ce7d0":"left"},"moduleLocationUpdate":{},"trashBinLocationUpdate":{"907ada4e-4996-4304-9844-b5c33858a582:trashBin":"cutoutA3"},"wasteChuteLocationUpdate":{},"stagingAreaLocationUpdate":{},"gripperLocationUpdate":{}},"930da113-1c57-44af-8674-4acfe9aef649":{"id":"930da113-1c57-44af-8674-4acfe9aef649","stepType":"moveLiquid","stepName":"transfer","stepDetails":"","stepNumber":0,"aspirate_airGap_checkbox":false,"aspirate_airGap_volume":"","aspirate_delay_checkbox":true,"aspirate_delay_seconds":"0.7","aspirate_flowRate":"200","aspirate_labware":"1ce7c748-a641-4285-811b-639ee5ad4b81:opentrons/agilent_1_reservoir_290ml/3","aspirate_mix_checkbox":false,"aspirate_mix_times":"1","aspirate_mix_volume":"50","aspirate_mmFromBottom":2,"aspirate_position_reference":"well-bottom","aspirate_retract_delay_seconds":"0","aspirate_retract_mmFromBottom":2,"aspirate_retract_speed":"10","aspirate_retract_x_position":0,"aspirate_retract_y_position":0,"aspirate_retract_position_reference":"well-top","aspirate_submerge_delay_seconds":"0","aspirate_submerge_speed":"10","aspirate_submerge_mmFromBottom":2,"aspirate_submerge_x_position":0,"aspirate_submerge_y_position":0,"aspirate_submerge_position_reference":"well-top","aspirate_touchTip_checkbox":false,"aspirate_touchTip_mmFromTop":-1,"aspirate_touchTip_speed":"30","aspirate_touchTip_mmFromEdge":"0.5","aspirate_wellOrder_first":"t2b","aspirate_wellOrder_second":"l2r","aspirate_wells_grouped":false,"aspirate_wells":["A1"],"aspirate_x_position":0,"aspirate_y_position":0,"blowout_checkbox":false,"blowout_flowRate":"223","blowout_location":"destination","changeTip":"always","conditioning_checkbox":false,"conditioning_volume":"","dispense_airGap_checkbox":false,"dispense_airGap_volume":"","dispense_delay_checkbox":true,"dispense_delay_seconds":"0.5","dispense_flowRate":"223","dispense_labware":"1ce7c748-a641-4285-811b-639ee5ad4b81:opentrons/agilent_1_reservoir_290ml/3","dispense_mix_checkbox":false,"dispense_mix_times":"1","dispense_mix_volume":"50","dispense_mmFromBottom":2,"dispense_position_reference":"well-bottom","dispense_retract_delay_seconds":"0","dispense_retract_mmFromBottom":2,"dispense_retract_speed":"10","dispense_retract_x_position":0,"dispense_retract_y_position":0,"dispense_retract_position_reference":"well-top","dispense_submerge_delay_seconds":"0","dispense_submerge_speed":"10","dispense_submerge_mmFromBottom":2,"dispense_submerge_x_position":0,"dispense_submerge_y_position":0,"dispense_submerge_position_reference":"well-top","dispense_touchTip_checkbox":false,"dispense_touchTip_mmFromTop":-1,"dispense_touchTip_speed":"30","dispense_touchTip_mmFromEdge":"0.5","dispense_wellOrder_first":"t2b","dispense_wellOrder_second":"l2r","dispense_wells":["A1"],"dispense_x_position":0,"dispense_y_position":0,"disposalVolume_checkbox":false,"disposalVolume_volume":"","dropTip_location":"907ada4e-4996-4304-9844-b5c33858a582:trashBin","liquidClassesSupported":true,"liquidClass":"glycerol50V1","nozzles":"ALL","path":"single","pipette":"77256c19-9f4f-4229-853e-3e2c6e1ce7d0","preWetTip":false,"pushOut_checkbox":true,"pushOut_volume":"35","tipRack":"opentrons/opentrons_flex_96_tiprack_1000ul/1","volume":"1000"}},"orderedStepIds":["930da113-1c57-44af-8674-4acfe9aef649"],"pipettes":{"77256c19-9f4f-4229-853e-3e2c6e1ce7d0":{"pipetteName":"p1000_96"}},"modules":{},"labware":{"5acddb58-a2b4-4d20-b9f7-fe6eeaeef5aa:opentrons/opentrons_flex_96_tiprack_adapter/1":{"displayName":"Opentrons Flex 96 Tip Rack Adapter","labwareDefURI":"opentrons/opentrons_flex_96_tiprack_adapter/1"},"3996b0b6-c6ad-4f3a-91e5-b680f37ee9ea:opentrons/opentrons_flex_96_tiprack_1000ul/1":{"displayName":"Opentrons Flex 96 Tip Rack 1000 µL","labwareDefURI":"opentrons/opentrons_flex_96_tiprack_1000ul/1"},"1ce7c748-a641-4285-811b-639ee5ad4b81:opentrons/agilent_1_reservoir_290ml/3":{"displayName":"Agilent 1 Well Reservoir 290 mL","labwareDefURI":"opentrons/agilent_1_reservoir_290ml/3"}}}},"metadata":{"protocolName":"","author":"","description":"","source":"Protocol Designer","created":1752007872902,"lastModified":1752009018918}}""" |
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Overview
Max flow-rate for T1000 at high volumes is 223 ul/sec, but the definitions say in places 250 ul/sec which is too high for ALL volumes. While lower target volumes cannot high 223 ul/sec (eg: 10 ul aspirate maxes out at <200 ul/sec), at least if we configure the default to be the max's max speed then we can tell a logic story for why it is that value.
Here's the 96ch water function with T1000 tips:
then my math to find that max flow-rate at 1000ul is:
Test Plan and Hands on Testing
Changelog
Review requests
Risk assessment
No risk. Does not change robot performance at all (because the hardware-controller was already limiting it silently).