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FlyResources.md

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Some helpful websites and tools for some in silico research on fly genes

FlyBase - Ties to together resources about the individual genes, including GBrowse gene locus viewer, predicted function according to InterPro and UniProt, lists of associated papers, etc.

FlyAtlas 2 – Gene expression profile, based on in situ hybridization (reliability?)

Virtual Fly Brain – Ties together transgenes/phenotypes/gene expression profile etc. for anatomical locations in the fly brain

Interactive fly – by Society for Developmental Biology, mostly anatomical and developmental resources on flies

Some helpful tools for the wet lab

FlyPrimerBank – Primers for almost every fly gene, some already tested and commented on (paper on FlyPrimerBank by Hu et al. 2013)

FlyC31 – Resources on PhiC31 integrase system implemented in flies

Crispr design tools

Zhang lab (also: Google discussion group) Crispr Design tool e-crisp

Further Crispr resources

OxfordFlyCrispr CrisprFlyDesign (also: Google discussion group)

Stock Centers

VDRC RNAi stock center – mainly RNAi lines and associated lines, e.g. Dicer2

Bloomington Fly Stock Center – like the default stock center w/ big selection, esp. of Gal4 driver lines

Miscellaneous

Addgene – extensive non-profit depository for plasmids/vectors, protocols, etc.

LifeTechnologies – Helpful How-To’s and Tips, e.g. Buffer Recipes

Cold spring Harbor Protocols