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I am simulating paired-end HiSeq reads from a single genome, and the insert sizes of the simulated reads are very different from what is expected from a HiSeq run. The insert sizes of the resulting reads seem to be evenly distributed between ~300-1200 bp (see here: simulated_insert_metric.pdf). Real sequencing data from HiSeq runs has insert sizes that peak around an expected value. I'm not sure what would be causing this.
The text was updated successfully, but these errors were encountered:
Do we have an update on this? I know the repo is quite old and maybe no one is managing it anymore, but a random insert size is not representative of a true dataset and can be a big issue for some softwares.
I am simulating paired-end HiSeq reads from a single genome, and the insert sizes of the simulated reads are very different from what is expected from a HiSeq run. The insert sizes of the resulting reads seem to be evenly distributed between ~300-1200 bp (see here: simulated_insert_metric.pdf). Real sequencing data from HiSeq runs has insert sizes that peak around an expected value. I'm not sure what would be causing this.
The text was updated successfully, but these errors were encountered: