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Unexpected distribution of insert sizes from paired-end HiSeq simulation #186

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allind opened this issue Aug 20, 2020 · 1 comment
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@allind
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allind commented Aug 20, 2020

I am simulating paired-end HiSeq reads from a single genome, and the insert sizes of the simulated reads are very different from what is expected from a HiSeq run. The insert sizes of the resulting reads seem to be evenly distributed between ~300-1200 bp (see here: simulated_insert_metric.pdf). Real sequencing data from HiSeq runs has insert sizes that peak around an expected value. I'm not sure what would be causing this.

@jsgounot
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jsgounot commented Apr 5, 2022

Do we have an update on this? I know the repo is quite old and maybe no one is managing it anymore, but a random insert size is not representative of a true dataset and can be a big issue for some softwares.

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