alphabetr implements the ALPHABETR (algorithm for pairing alpha-beta T cell receptors) algorithms for obtaining TCR sequence pairs. This approach determines CDR3A/CDR3B pairs from high-throughput sequencing data from repeated samples of antigen-specific T cell populations.
With alphabetr, you can
- Determine CDR3A/CDR3B pairs
- Determine dual TCR-alpha clones and clones that share CDR3A or CDR3B sequences
- Estimate clonal frequencies
You can install
- the latest version released on CRAN with
install.packages("alphabetr")- the latest development version found on github with
if (packageVersion("devtools") < 1.6) {
install.packages("devtools")
}
devtools::install_github("edwardslee/alphabetr")If you encounter any bugs, please file an issue.
Please see our paper in PLOS Computational Biology for more details about the algorithm and how it was tested (DOI: 10.1371/journal.pcbi.1005313).