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Error while running extractHAIRS #127

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uncleflybfchen opened this issue Nov 16, 2022 · 0 comments
Open

Error while running extractHAIRS #127

uncleflybfchen opened this issue Nov 16, 2022 · 0 comments

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@uncleflybfchen
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When running extractHAIRS, if the GT of the first sample is a missing value (i.e. . /.) then the following error message will appear. But if the GT of the other samples is a missing value, there is no error.
Is there any solution to this problem? Thanks.

ERROR: Non-diploid VCF entry detected. Each VCF entry must have a diploid genotype (GT) field consisting of two alleles in the set {0,1,2} separated by either '/' or '|'. For example, "1/1", "0/1", and "0|2" are valid diploid genotypes for HapCUT2, but "1", "0/3", and "0/0/1" are not.
The invalid entry is:

NNEMv4.scaff0001        241517  DEL00000145     A       <DEL>   424     PASS    PRECISE;SVTYPE=DEL;SVMETHOD=EMBL.DELLYv1.1.5;END=241557;PE=0;MAPQ=0;CT=3to5;CIPOS=-3,3;CIEND=-3,3;SRMAPQ=60;INSLEN=0;HOMLEN=4;SR=8;SRQ=0.994898;CONSENSUS=TTTTATATTAATCAGCGATTTATTGCCGATTTTTTGGTATATAAATGATTACGCAATCTTGATTACGAACGGAGATATGACATTTTTCGCAATTGCTCGAAAACAGCGATTTATTGCCGATTTTTTGGTATATAAACGATTGCGCTATCTTGATTAGGAACGAAGATATGATGTTTTTCGCAATTGCTCGGAAACG;CE=1.9088;RDRATIO=0.28128        GT:GL:GQ:FT:RCL:RC:RCR:RDCN:DR:DV:RR:RV ./.:0,0,0:0:LowQual:0:20:49:1:0:0:0:0   0/0:0,-0.50527,-4.10321:6:LowQual:20:117:114:2:0:0:2:0  0/0:0,-1.49882,-15.7937:15:PASS:36:270:277:2:0:0:5:0    ./.:0,0,0:0:LowQual:0:39:54:1:0:0:0:0   0/0:0,-0.806213,-7.80312:9:LowQual:26:155:162:2:0:0:3:0 0/0:0,-0.300893,-3.49986:5:LowQual:0:70:99:1:0:0:1:0    ./.:0,0,0:0:LowQual:0:0:15:0:0:0:0:0    0/0:0,-0.601756,-6.9997:7:LowQual:10:152:103:3:0:0:2:0  0/0:0,-0.278699,-1.27767:4:LowQual:8:91:80:2:0:0:1:0    0/0:0,-0.601084,-6.29902:7:LowQual:20:136:158:2:0:0:2:0 0/0:0,-0.566029,-4.76397:7:LowQual:12:89:66:2:0:0:2:0   0/0:0,-0.300921,-3.59989:5:LowQual:7:17:20:1:0:0:1:0    0/0:0,-0.50454,-3.30248:6:LowQual:4:86:77:2:0:0:2:0
     0/0:0,-0.300943,-3.69991:5:LowQual:0:110:113:2:0:0:1:0  ./.:0,0,0:0:LowQual:17:17:89:0:0:0:0:0  0/0:0,-0.902319,-10.0992:10:LowQual:0:200:248:2:0:0:3:0 0/0:0,-0.300921,-3.59989:5:LowQual:16:29:40:1:0:0:1:0   0/0:0,-1.2037,-14.4996:12:LowQual:11:230:189:2:0:0:4:0  0/0:0,-0.300921,-3.59989:5:LowQual:0:31:94:1:0:0:1:0    0/0:0,-0.601864,-7.2998:7:LowQual:0:93:117:2:0:0:2:0    0/0:0,-1.67824,-16.3721:17:PASS:279:469:235:2:0:0:6:0   0/0:0,-0.175402,-0.474372:3:LowQual:20:23:47:1:0:0:1:0  0/0:0,-4.20711,-48.5927:42:PASS:531:849:360:2:0:0:14:0  0/1:-60.7798,0,-1.89853:19:PASS:380:203:391:1:0:0:3:19  0/0:0,-2.10601,-24.3988:21:PASS:198:394:267:2:0:0:7:0   0/0:0,-1.50377,-17.7986:15:PASS:232:362:214:2:0:0:5:0   0/0:0,-1.51483,-15.3087:15:PASS:166:525:320:2:0:0:6:0   0/0:0,-1.14241,-9.73829:11:LowQual:354:540:214:2:0:0:4:0        0/0:0,-1.20118,-13.6971:12:LowQual:108:302:173:2:0:0:4:0        0/0:0,-1.17887,-10.2748:12:LowQual:98:226:205:1:0:0:4:0 0/0:0,-1.80548,-21.4993:18:PASS:233:329:128:2:0:0:6:0   0/0:0,-1.71393,-15.7077:17:PASS:180:415:286:2:0:0:6:0   0/0:0,-1.80008,-19.6939:18:PASS:183:502:310:2:0:0:6:0   0/0:0,-1.18174,-10.1776:12:LowQual:128:228:127:2:0:0:4:0        0/0:0,-2.08898,-23.2818:21:PASS:519:560:133:2:0:0:7:0   0/0:0,-1.79998,-19.5938:18:PASS:224:286:130:2:0:0:6:0   0/0:0,-2.8441,-32.5338:28:PASS:555:812:265:2:0:0:10:0   0/0:0,-2.54294,-28.5337:25:PASS:330:613:264:2:0:0:9:0   0/0:0,-1.47987,-11.9747:15:PASS:345:485:121:2:0:0:5:0   0/0:0,-2.40734,-28.7991:24:PASS:322:494:162:2:0:0:8:0   0/0:0,-2.7029,-29.4936:27:PASS:455:617:254:2:0:0:9:0    0/0:0,-4.503,-49.5876:45:PASS:708:973:365:2:0:0:15:0    0/0:0,-1.20371,-14.4996:12:LowQual:208:263:102:2:0:0:4:0        0/0:0,-1.80202,-19.5958:18:PASS:185:402:211:2:0:0:6:0   0/0:0,-0.902757,-10.7997:10:LowQual:74:125:80:2:0:0:3:0 0/0:0,-4.25117,-39.6357:43:PASS:465:837:405:2:0:0:15:0  0/0:0,-2.1063,-24.7991:21:PASS:209:351:185:2:0:0:7:0    0/0:0,-2.40733,-28.6991:24:PASS:338:631:294:2:0:0:8:0   0/0:0,-6.81217,-75.5885:68:PASS:1750:1961:517:2:0:0:23:0        ./.:0,0,0:0:LowQual:252:1109:710:2:0:0:0:0      0/0:0,-4.20711,-48.0927:42:PASS:625:952:441:2:0:0:14:0  0/0:0,-2.40263,-24.6944:24:PASS:446:536:130:2:0:0:8:0   0/0:0,-0.776,-6.67291:9:LowQual:104:151:40:2:0:0:3:0    0/0:0,-1.19274,-12.3886:12:LowQual:387:530:180:2:0:0:4:0        0/0:0,-1.20232,-12.5982:12:LowQual:102:283:193:2:0:0:4:0        0/0:0,-1.19685,-12.5927:12:LowQual:227:473:211:2:0:0:4:0        0/0:0,-3.00921,-36.0989:30:PASS:318:527:245:2:0:0:10:0  0/0:0,-2.40716,-28.2989:24:PASS:323:596:300:2:0:0:8:0   0/0:0,-11.1336,-132.496:111:PASS:1143:2164:1075:2:0:0:37:0      0/0:0,-0.300893,-3.49986:5:LowQual:67:153:82:2:0:0:1:0  0/0:0,-1.50446,-17.5993:15:PASS:117:341:210:2:0:0:5:0   0/0:0,-0.6018,-7.09974:7:LowQual:71:229:133:2:0:0:2:0   0/0:0,-2.10641,-25.1992:21:PASS:111:413:250:2:0:0:7:0   0/0:0,-0.300943,-3.69991:5:LowQual:50:165:140:2:0:0:1:0 0/0:0,-1.50451,-17.7994:15:PASS:127:258:146:2:0:0:5:0   0/0:0,-2.38493,-25.9767:24:PASS:359:555:238:2:0:0:8:0   0/0:0,-4.20878,-49.4944:42:PASS:369:725:392:2:0:0:14:0  0/0:0,-3.00843,-34.1981:30:PASS:302:549:277:2:0:0:10:0
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