Relion 5 fails to reconstruct tomograms by asking for _rlnTomoMicrographNameEven label

[Amy1809]

Describe your problem
I am following the Relion pipeline starting from import - motion correction - CTF estimation. I have passed the tilt series alignment stage using AreTomo in Relion and now want to reconstruct tomograms. Here is my full command:
which relion_tomo_reconstruct_tomogram --t AlignTiltSeries/job028/aligned_tilt_series.star --o Tomograms/job033/ --w 4200 --h 4200 --d 300 --binned_angpix 10 --fourier --ctf_intact_first_peak --do_proj --centre_proj 0 --thickness_proj 10 --tiltangle_offset -2 --only_do_unfinished --j 1 --pipeline_control Tomograms/job033/

ERROR: tomogramTable for Position_1 does not contain a rlnTomoMicrographNameEven label

Here is my input file from the tilt series alignment 'Aligned_tilt_series.star':

# version 50001

data_Position_1

loop_ 
_rlnMicrographMovieName #1 
_rlnTomoTiltMovieFrameCount #2 
_rlnTomoNominalStageTiltAngle #3 
_rlnTomoNominalTiltAxisAngle #4 
_rlnMicrographPreExposure #5 
_rlnTomoNominalDefocus #6 
_rlnCtfPowerSpectrum #7 
_rlnMicrographName #8 
_rlnMicrographMetadata #9 
_rlnAccumMotionTotal #10 
_rlnAccumMotionEarly #11 
_rlnAccumMotionLate #12 
_rlnCtfImage #13 
_rlnDefocusU #14 
_rlnDefocusV #15 
_rlnCtfAstigmatism #16 
_rlnDefocusAngle #17 
_rlnCtfFigureOfMerit #18 
_rlnCtfMaxResolution #19 
_rlnCtfIceRingDensity #20 
test_file/Position_1_001_12.00_20240509_231312_Fractions.mrc            6    11.970000    69.000000     0.000000     -5.00000 MotionCorr/job021/test_file/Position_1_001_12_00_20240509_231312_Fractions_PS.mrc MotionCorr/job021/test_file/Position_1_001_12_00_20240509_231312_Fractions.mrc MotionCorr/job021/test_file/Position_1_001_12_00_20240509_231312_Fractions.star     7.038247     4.829588     2.208659 CtfFind/job023/test_file/Position_1_001_12_00_20240509_231312_Fractions_PS.ctf:mrc 50098.101562 49296.847656   801.253906    -15.76935     0.049158     6.900869     0.985160 
test_file/Position_1_002_15.00_20240509_231404_Fractions.mrc            6    14.980000    69.000000     3.000000     -5.00000 MotionCorr/job021/test_file/Position_1_002_15_00_20240509_231404_Fractions_PS.mrc MotionCorr/job021/test_file/Position_1_002_15_00_20240509_231404_Fractions.mrc MotionCorr/job021/test_file/Position_1_002_15_00_20240509_231404_Fractions.star     5.974551     0.000000     5.974551 CtfFind/job023/test_file/Position_1_002_15_00_20240509_231404_Fractions_PS.ctf:mrc 49646.906250 49262.035156   384.871094    18.592125     0.051769     7.149549     0.137120 
test_file/Position_1_003_18.00_20240509_231510_Fractions.mrc            6    17.980000    69.000000     6.000000     -5.00000 MotionCorr/job021/test_file/Position_1_003_18_00_20240509_231510_Fractions_PS.mrc MotionCorr/job021/test_file/Position_1_003_18_00_20240509_231510_Fractions.mrc MotionCorr/job021/test_file/Position_1_003_18_00_20240509_231510_Fractions.star     6.324707     0.000000     6.324707 CtfFind/job023/test_file/Position_1_003_18_00_20240509_231510_Fractions_PS.ctf:mrc 49754.980469 49373.078125   381.902344     2.922433     0.052668     7.416822     0.783220 
test_file/Position_1_004_9.00_20240509_231616_Fractions.mrc            6     8.980000    69.000000     9.000000     -5.00000 MotionCorr/job021/test_file/Position_1_004_9_00_20240509_231616_Fractions_PS.mrc MotionCorr/job021/test_file/Position_1_004_9_00_20240509_231616_Fractions.mrc MotionCorr/job021/test_file/Position_1_004_9_00_20240509_231616_Fractions.star     7.091226     0.000000     7.091226 CtfFind/job023/test_file/Position_1_004_9_00_20240509_231616_Fractions_PS.ctf:mrc 50158.078125 49471.324219   686.753906     1.242941     0.047665     7.149549     0.889940 
test_file/Position_1_005_6.00_20240509_231721_Fractions.mrc            6     5.980000    69.000000    12.000000     -5.00000 MotionCorr/job021/test_file/Position_1_005_6_00_20240509_231721_Fractions_PS.mrc MotionCorr/job021/test_file/Position_1_005_6_00_20240509_231721_Fractions.mrc MotionCorr/job021/test_file/Position_1_005_6_00_20240509_231721_Fractions.star     6.930793     0.000000     6.930793 CtfFind/job023/test_file/Position_1_005_6_00_20240509_231721_Fractions_PS.ctf:mrc 49756.562500 49415.101562   341.460938     3.844380     0.046210     7.348148     0.474730 
test_file/Position_1_006_3.00_20240509_231827_Fractions.mrc            6     2.980000    69.000000    15.000000     -5.00000 MotionCorr/job021/test_file/Position_1_006_3_00_20240509_231827_Fractions_PS.mrc MotionCorr/job021/test_file/Position_1_006_3_00_20240509_231827_Fractions.mrc MotionCorr/job021/test_file/Position_1_006_3_00_20240509_231827_Fractions.star     7.253323     0.000000     7.253323 CtfFind/job023/test_file/Position_1_006_3_00_20240509_231827_Fractions_PS.ctf:mrc 50025.136719 49353.226562   671.910156    -22.66424     0.049492     7.416822     0.208050 
test_file/Position_1_007_21.00_20240509_231934_Fractions.mrc            6    20.980000    69.000000    18.000000     -5.00000 MotionCorr/job021/test_file/Position_1_007_21_00_20240509_231934_Fractions_PS.mrc MotionCorr/job021/test_file/Position_1_007_21_00_20240509_231934_Fractions.mrc MotionCorr/job021/test_file/Position_1_007_21_00_20240509_231934_Fractions.star     6.539367     0.000000     6.539367 CtfFind/job023/test_file/Position_1_007_21_00_20240509_231934_Fractions_PS.ctf:mrc 49816.671875 49010.195312   806.476562     -1.47460     0.058071     6.504918     0.616830 
test_file/Position_1_008_24.00_20240509_232041_Fractions.mrc            6    23.990000    69.000000    21.000000     -5.00000 MotionCorr/job021/test_file/Position_1_008_24_00_20240509_232041_Fractions_PS.mrc MotionCorr/job021/test_file/Position_1_008_24_00_20240509_232041_Fractions.mrc MotionCorr/job021/test_file/Position_1_008_24_00_20240509_232041_Fractions.star     6.303729     0.000000     6.303729 CtfFind/job023/test_file/Position_1_008_24_00_20240509_232041_Fractions_PS.ctf:mrc 49892.230469 48896.503906   995.726562     5.630313     0.061713     6.900869     0.213240 
test_file/Position_1_009_27.00_20240509_232145_Fractions.mrc            6    26.990000    69.000000    24.000000     -5.00000 MotionCorr/job021/test_file/Position_1_009_27_00_20240509_232145_Fractions_PS.mrc MotionCorr/job021/test_file/Position_1_009_27_00_20240509_232145_Fractions.mrc MotionCorr/job021/test_file/Position_1_009_27_00_20240509_232145_Fractions.star     6.087909     0.000000     6.087909 CtfFind/job023/test_file/Position_1_009_27_00_20240509_232145_Fractions_PS.ctf:mrc 49877.195312 49234.203125   642.992188     -6.54559     0.059930     6.668907     0.445060 
test_file/Position_1_010_0.00_20240509_232253_Fractions.mrc            6     -0.02000    69.000000    27.000000     -5.00000 MotionCorr/job021/test_file/Position_1_010_0_00_20240509_232253_Fractions_PS.mrc MotionCorr/job021/test_file/Position_1_010_0_00_20240509_232253_Fractions.mrc MotionCorr/job021/test_file/Position_1_010_0_00_20240509_232253_Fractions.star     7.644732     0.000000     7.644732 CtfFind/job023/test_file/Position_1_010_0_00_20240509_232253_Fractions_PS.ctf:mrc 50718.019531 49157.617188  1560.402344     -2.61935     0.046556     6.841379     0.337580 
test_file/Position_1_011_-3.00_20240509_232359_Fractions.mrc            6     -3.02000    69.000000    30.000000     -5.00000 MotionCorr/job021/test_file/Position_1_011_-3_00_20240509_232359_Fractions_PS.mrc MotionCorr/job021/test_file/Position_1_011_-3_00_20240509_232359_Fractions.mrc MotionCorr/job021/test_file/Position_1_011_-3_00_20240509_232359_Fractions.star     7.684926     0.000000     7.684926 CtfFind/job023/test_file/Position_1_011_-3_00_20240509_232359_Fractions_PS.ctf:mrc 50292.203125 49649.960938   642.242188    61.986439     0.033017     8.181443     0.788410 
test_file/Position_1_012_-6.00_20240509_232505_Fractions.mrc            6     -6.02000    69.000000    33.000000     -5.00000 MotionCorr/job021/test_file/Position_1_012_-6_00_20240509_232505_Fractions_PS.mrc MotionCorr/job021/test_file/Position_1_012_-6_00_20240509_232505_Fractions.mrc MotionCorr/job021/test_file/Position_1_012_-6_00_20240509_232505_Fractions.star     7.919336     0.000000     7.919336 CtfFind/job023/test_file/Position_1_012_-6_00_20240509_232505_Fractions_PS.ctf:mrc 49802.667969 49408.824219   393.843750    -11.43185     0.023930     8.353684     0.767390 
test_file/Position_1_013_30.00_20240509_232615_Fractions.mrc            6    29.980000    69.000000    36.000000     -5.00000 MotionCorr/job021/test_file/Position_1_013_30_00_20240509_232615_Fractions_PS.mrc MotionCorr/job021/test_file/Position_1_013_30_00_20240509_232615_Fractions.mrc MotionCorr/job021/test_file/Position_1_013_30_00_20240509_232615_Fractions.star     6.487063     0.000000     6.487063 CtfFind/job023/test_file/Position_1_013_30_00_20240509_232615_Fractions_PS.ctf:mrc 49847.320312 49447.218750   400.101562    -10.98404     0.051925     8.817778     1.655500 
test_file/Position_1_014_33.00_20240509_232719_Fractions.mrc            6    32.980000    69.000000    39.000000     -5.00000 MotionCorr/job021/test_file/Position_1_014_33_00_20240509_232719_Fractions_PS.mrc MotionCorr/job021/test_file/Position_1_014_33_00_20240509_232719_Fractions.mrc MotionCorr/job021/test_file/Position_1_014_33_00_20240509_232719_Fractions.star     5.879057     0.000000     5.879057 CtfFind/job023/test_file/Position_1_014_33_00_20240509_232719_Fractions_PS.ctf:mrc 49921.324219 49014.851562   906.472656    -12.16602     0.043564     7.857426     0.709480 
test_file/Position_1_015_36.00_20240509_232824_Fractions.mrc            6    35.990000    69.000000    42.000000     -5.00000 MotionCorr/job021/test_file/Position_1_015_36_00_20240509_232824_Fractions_PS.mrc MotionCorr/job021/test_file/Position_1_015_36_00_20240509_232824_Fractions.mrc MotionCorr/job021/test_file/Position_1_015_36_00_20240509_232824_Fractions.star     4.726918     0.000000     4.726918 CtfFind/job023/test_file/Position_1_015_36_00_20240509_232824_Fractions_PS.ctf:mrc 50157.988281 49520.167969   637.820312    -19.70231     0.056110     7.780392     1.285000 
test_file/Position_1_016_-9.00_20240509_232933_Fractions.mrc            6     -9.02000    69.000000    45.000000     -5.00000 MotionCorr/job021/test_file/Position_1_016_-9_00_20240509_232933_Fractions_PS.mrc MotionCorr/job021/test_file/Position_1_016_-9_00_20240509_232933_Fractions.mrc MotionCorr/job021/test_file/Position_1_016_-9_00_20240509_232933_Fractions.star     9.319104     0.000000     9.319104 CtfFind/job023/test_file/Position_1_016_-9_00_20240509_232933_Fractions_PS.ctf:mrc 50711.792969 49869.730469   842.062500    -18.78392     0.037172     8.817778     1.699140 
test_file/Position_1_017_-12.00_20240509_233041_Fractions.mrc            6    -12.02000    69.000000    48.000000     -5.00000 MotionCorr/job021/test_file/Position_1_017_-12_00_20240509_233041_Fractions_PS.mrc MotionCorr/job021/test_file/Position_1_017_-12_00_20240509_233041_Fractions.mrc MotionCorr/job021/test_file/Position_1_017_-12_00_20240509_233041_Fractions.star     9.020844     0.000000     9.020844 CtfFind/job023/test_file/Position_1_017_-12_00_20240509_233041_Fractions_PS.ctf:mrc 50538.359375 49761.839844   776.519531     1.552089     0.036976     8.916854     1.429070 
test_file/Position_1_018_-15.00_20240509_233147_Fractions.mrc            6    -15.02000    69.000000    51.000000     -5.00000 MotionCorr/job021/test_file/Position_1_018_-15_00_20240509_233147_Fractions_PS.mrc MotionCorr/job021/test_file/Position_1_018_-15_00_20240509_233147_Fractions.mrc MotionCorr/job021/test_file/Position_1_018_-15_00_20240509_233147_Fractions.star     8.622630     0.000000     8.622630 CtfFind/job023/test_file/Position_1_018_-15_00_20240509_233147_Fractions_PS.ctf:mrc 50480.621094 49827.656250   652.964844    -10.83642     0.034775     9.797531     2.087490 
test_file/Position_1_019_39.00_20240509_233256_Fractions.mrc            6    38.990000    69.000000    54.000000     -5.00000 MotionCorr/job021/test_file/Position_1_019_39_00_20240509_233256_Fractions_PS.mrc MotionCorr/job021/test_file/Position_1_019_39_00_20240509_233256_Fractions.mrc MotionCorr/job021/test_file/Position_1_019_39_00_20240509_233256_Fractions.star    20.046418     0.000000    20.046418 CtfFind/job023/test_file/Position_1_019_39_00_20240509_233256_Fractions_PS.ctf:mrc 50712.593750 50549.242188   163.351562    -49.26703     0.020279    11.844776     2.311650 
test_file/Position_1_020_42.00_20240509_233400_Fractions.mrc            6    41.980000    69.000000    57.000000     -5.00000 MotionCorr/job021/test_file/Position_1_020_42_00_20240509_233400_Fractions_PS.mrc MotionCorr/job021/test_file/Position_1_020_42_00_20240509_233400_Fractions.mrc MotionCorr/job021/test_file/Position_1_020_42_00_20240509_233400_Fractions.star    37.102423     0.000000    37.102423 CtfFind/job023/test_file/Position_1_020_42_00_20240509_233400_Fractions_PS.ctf:mrc 59420.738281 59201.871094   218.867188    16.747705     0.024057     9.121839     1.359120 
test_file/Position_1_021_45.00_20240509_233505_Fractions.mrc            6    44.980000    69.000000    60.000000     -5.00000 MotionCorr/job021/test_file/Position_1_021_45_00_20240509_233505_Fractions_PS.mrc MotionCorr/job021/test_file/Position_1_021_45_00_20240509_233505_Fractions.mrc MotionCorr/job021/test_file/Position_1_021_45_00_20240509_233505_Fractions.star    23.401099     0.000000    23.401099 CtfFind/job023/test_file/Position_1_021_45_00_20240509_233505_Fractions_PS.ctf:mrc 44078.625000 43359.250000   719.375000    -37.25490     0.039790     9.227907     1.784920 
test_file/Position_1_022_-18.00_20240509_233620_Fractions.mrc            6    -18.01000    69.000000    63.000000     -5.00000 MotionCorr/job021/test_file/Position_1_022_-18_00_20240509_233620_Fractions_PS.mrc MotionCorr/job021/test_file/Position_1_022_-18_00_20240509_233620_Fractions.mrc MotionCorr/job021/test_file/Position_1_022_-18_00_20240509_233620_Fractions.star     7.937368     0.000000     7.937368 CtfFind/job023/test_file/Position_1_022_-18_00_20240509_233620_Fractions_PS.ctf:mrc 50409.152344 49895.722656   513.429688    -12.53511     0.048772     9.797531     1.711280 
test_file/Position_1_023_-21.00_20240509_233726_Fractions.mrc            6    -21.01000    69.000000    66.000000     -5.00000 MotionCorr/job021/test_file/Position_1_023_-21_00_20240509_233726_Fractions_PS.mrc MotionCorr/job021/test_file/Position_1_023_-21_00_20240509_233726_Fractions.mrc MotionCorr/job021/test_file/Position_1_023_-21_00_20240509_233726_Fractions.star     8.571939     0.000000     8.571939 CtfFind/job023/test_file/Position_1_023_-21_00_20240509_233726_Fractions_PS.ctf:mrc 51047.359375 50283.718750   763.640625    -41.00784     0.052649    11.022222     2.034580 
test_file/Position_1_024_-24.00_20240509_233832_Fractions.mrc            6    -24.01000    69.000000    69.000000     -5.00000 MotionCorr/job021/test_file/Position_1_024_-24_00_20240509_233832_Fractions_PS.mrc MotionCorr/job021/test_file/Position_1_024_-24_00_20240509_233832_Fractions.mrc MotionCorr/job021/test_file/Position_1_024_-24_00_20240509_233832_Fractions.star     8.590813     0.000000     8.590813 CtfFind/job023/test_file/Position_1_024_-24_00_20240509_233832_Fractions_PS.ctf:mrc 51249.125000 50781.742188   467.382812    77.489174     0.021558    11.844776     2.232300 
test_file/Position_1_025_48.00_20240509_233942_Fractions.mrc            6    47.990000    69.000000    72.000000     -5.00000 MotionCorr/job021/test_file/Position_1_025_48_00_20240509_233942_Fractions_PS.mrc MotionCorr/job021/test_file/Position_1_025_48_00_20240509_233942_Fractions.mrc MotionCorr/job021/test_file/Position_1_025_48_00_20240509_233942_Fractions.star     3.584342     0.000000     3.584342 CtfFind/job023/test_file/Position_1_025_48_00_20240509_233942_Fractions_PS.ctf:mrc 47698.019531 47489.039062   208.980469    -50.36451     0.022499    10.581333     0.861630 
test_file/Position_1_026_51.00_20240509_234048_Fractions.mrc            6    50.990000    69.000000    75.000000     -5.00000 MotionCorr/job021/test_file/Position_1_026_51_00_20240509_234048_Fractions_PS.mrc MotionCorr/job021/test_file/Position_1_026_51_00_20240509_234048_Fractions.mrc MotionCorr/job021/test_file/Position_1_026_51_00_20240509_234048_Fractions.star   306.037718     0.000000   306.037718 CtfFind/job023/test_file/Position_1_026_51_00_20240509_234048_Fractions_PS.ctf:mrc 50717.808594 50481.105469   236.703125    -29.73265     0.026517     9.678048     0.488150 
test_file/Position_1_027_54.00_20240509_234216_Fractions.mrc            6    53.990000    69.000000    78.000000     -5.00000 MotionCorr/job021/test_file/Position_1_027_54_00_20240509_234216_Fractions_PS.mrc MotionCorr/job021/test_file/Position_1_027_54_00_20240509_234216_Fractions.mrc MotionCorr/job021/test_file/Position_1_027_54_00_20240509_234216_Fractions.star   255.568175     0.000000   255.568175 CtfFind/job023/test_file/Position_1_027_54_00_20240509_234216_Fractions_PS.ctf:mrc 48096.351562 47890.019531   206.332031    -74.53869     0.005632    12.596825     0.650530 
test_file/Position_1_028_-27.00_20240509_234334_Fractions.mrc            6    -27.01000    69.000000    81.000000     -5.00000 MotionCorr/job021/test_file/Position_1_028_-27_00_20240509_234334_Fractions_PS.mrc MotionCorr/job021/test_file/Position_1_028_-27_00_20240509_234334_Fractions.mrc MotionCorr/job021/test_file/Position_1_028_-27_00_20240509_234334_Fractions.star    10.671405     0.000000    10.671405 CtfFind/job023/test_file/Position_1_028_-27_00_20240509_234334_Fractions_PS.ctf:mrc 49188.593750 49135.882812    52.710938    26.986500     0.050372    11.501449     2.307180 
test_file/Position_1_029_-30.00_20240509_234440_Fractions.mrc            6    -30.01000    69.000000    84.000000     -5.00000 MotionCorr/job021/test_file/Position_1_029_-30_00_20240509_234440_Fractions_PS.mrc MotionCorr/job021/test_file/Position_1_029_-30_00_20240509_234440_Fractions.mrc MotionCorr/job021/test_file/Position_1_029_-30_00_20240509_234440_Fractions.star    12.111194     0.000000    12.111194 CtfFind/job023/test_file/Position_1_029_-30_00_20240509_234440_Fractions_PS.ctf:mrc 49964.378906 49835.816406   128.562500    -55.70428     0.064729    11.022222     1.811510 
test_file/Position_1_030_-33.00_20240509_234548_Fractions.mrc            6    -33.01000    69.000000    87.000000     -5.00000 MotionCorr/job021/test_file/Position_1_030_-33_00_20240509_234548_Fractions_PS.mrc MotionCorr/job021/test_file/Position_1_030_-33_00_20240509_234548_Fractions.mrc MotionCorr/job021/test_file/Position_1_030_-33_00_20240509_234548_Fractions.star    12.936012     0.000000    12.936012 CtfFind/job023/test_file/Position_1_030_-33_00_20240509_234548_Fractions_PS.ctf:mrc 49512.796875 49486.207031    26.589844    -13.32284     0.057942    12.400000     1.319840 
test_file/Position_1_031_57.00_20240509_234723_Fractions.mrc            6    56.990000    69.000000    90.000000     -5.00000 MotionCorr/job021/test_file/Position_1_031_57_00_20240509_234723_Fractions_PS.mrc MotionCorr/job021/test_file/Position_1_031_57_00_20240509_234723_Fractions.mrc MotionCorr/job021/test_file/Position_1_031_57_00_20240509_234723_Fractions.star   148.009082     0.000000   148.009082 CtfFind/job023/test_file/Position_1_031_57_00_20240509_234723_Fractions_PS.ctf:mrc 50901.515625 50811.257812    90.257812    26.028786     0.037464     9.121839     1.427140 
test_file/Position_1_032_60.00_20240509_234853_Fractions.mrc            6    59.990000    69.000000    93.000000     -5.00000 MotionCorr/job021/test_file/Position_1_032_60_00_20240509_234853_Fractions_PS.mrc MotionCorr/job021/test_file/Position_1_032_60_00_20240509_234853_Fractions.mrc MotionCorr/job021/test_file/Position_1_032_60_00_20240509_234853_Fractions.star   263.435121     0.000000   263.435121 CtfFind/job023/test_file/Position_1_032_60_00_20240509_234853_Fractions_PS.ctf:mrc 51555.183594 51513.878906    41.304688    -32.73188     0.016191    25.000000     1.165240 
test_file/Position_1_033_63.00_20240509_235025_Fractions.mrc            6    62.990000    69.000000    96.000000     -5.00000 MotionCorr/job021/test_file/Position_1_033_63_00_20240509_235025_Fractions_PS.mrc MotionCorr/job021/test_file/Position_1_033_63_00_20240509_235025_Fractions.mrc MotionCorr/job021/test_file/Position_1_033_63_00_20240509_235025_Fractions.star   256.290656     0.000000   256.290656 CtfFind/job023/test_file/Position_1_033_63_00_20240509_235025_Fractions_PS.ctf:mrc 49798.960938 49609.703125   189.257812     9.414087     0.022227     9.447619     0.641490 
test_file/Position_1_034_-36.00_20240509_235139_Fractions.mrc            6    -36.00000    69.000000    99.000000     -5.00000 MotionCorr/job021/test_file/Position_1_034_-36_00_20240509_235139_Fractions_PS.mrc MotionCorr/job021/test_file/Position_1_034_-36_00_20240509_235139_Fractions.mrc MotionCorr/job021/test_file/Position_1_034_-36_00_20240509_235139_Fractions.star    17.542048     0.000000    17.542048 CtfFind/job023/test_file/Position_1_034_-36_00_20240509_235139_Fractions_PS.ctf:mrc 48683.472656 48433.195312   250.277344    36.912773     0.066547    14.696296     2.191390 
test_file/Position_1_035_-39.00_20240509_235245_Fractions.mrc            6    -39.00000    69.000000   102.000000     -5.00000 MotionCorr/job021/test_file/Position_1_035_-39_00_20240509_235245_Fractions_PS.mrc MotionCorr/job021/test_file/Position_1_035_-39_00_20240509_235245_Fractions.mrc MotionCorr/job021/test_file/Position_1_035_-39_00_20240509_235245_Fractions.star    18.387375     0.000000    18.387375 CtfFind/job023/test_file/Position_1_035_-39_00_20240509_235245_Fractions_PS.ctf:mrc 49642.199219 49388.335938   253.863281    24.099264     0.057095    15.261538     2.089740 
test_file/Position_1_036_-42.00_20240509_235352_Fractions.mrc            6    -42.00000    69.000000   105.000000     -5.00000 MotionCorr/job021/test_file/Position_1_036_-42_00_20240509_235352_Fractions_PS.mrc MotionCorr/job021/test_file/Position_1_036_-42_00_20240509_235352_Fractions.mrc MotionCorr/job021/test_file/Position_1_036_-42_00_20240509_235352_Fractions.star    18.812348     0.000000    18.812348 CtfFind/job023/test_file/Position_1_036_-42_00_20240509_235352_Fractions_PS.ctf:mrc 48916.121094 48790.464844   125.656250    -83.07788     0.044530    14.973584     2.101310 
test_file/Position_1_037_66.00_20240509_235530_Fractions.mrc            6    65.990000    69.000000   108.000000     -5.00000 MotionCorr/job021/test_file/Position_1_037_66_00_20240509_235530_Fractions_PS.mrc MotionCorr/job021/test_file/Position_1_037_66_00_20240509_235530_Fractions.mrc MotionCorr/job021/test_file/Position_1_037_66_00_20240509_235530_Fractions.star   254.708206     0.000000   254.708206 CtfFind/job023/test_file/Position_1_037_66_00_20240509_235530_Fractions_PS.ctf:mrc 49889.828125 49856.273438    33.554688    -43.95515     0.009299    25.000000     1.646930 
test_file/Position_1_038_69.00_20240509_235700_Fractions.mrc            6    68.990000    69.000000   111.000000     -5.00000 MotionCorr/job021/test_file/Position_1_038_69_00_20240509_235700_Fractions_PS.mrc MotionCorr/job021/test_file/Position_1_038_69_00_20240509_235700_Fractions.mrc MotionCorr/job021/test_file/Position_1_038_69_00_20240509_235700_Fractions.star   192.860005     0.000000   192.860005 CtfFind/job023/test_file/Position_1_038_69_00_20240509_235700_Fractions_PS.ctf:mrc 56528.105469 56382.695312   145.410156    -36.51537     0.058625    15.560784     0.671490 
test_file/Position_1_039_-45.00_20240509_235801_Fractions.mrc            6    -45.01000    69.000000   114.000000     -5.00000 MotionCorr/job021/test_file/Position_1_039_-45_00_20240509_235801_Fractions_PS.mrc MotionCorr/job021/test_file/Position_1_039_-45_00_20240509_235801_Fractions.mrc MotionCorr/job021/test_file/Position_1_039_-45_00_20240509_235801_Fractions.star    21.964691     0.000000    21.964691 CtfFind/job023/test_file/Position_1_039_-45_00_20240509_235801_Fractions_PS.ctf:mrc 49001.250000 48973.769531    27.480469    -66.37075     0.051799    14.973584     0.190490 

I can see that I am missing the _rlnTomoMicrographNameEven label, but this lable is not generated from my previous .star file during the motion correction and CTF estimation stage. I have looked at other people's file and know that Relion is looking for file similar to 'MotionCorr/job002/frames/ts_00105_45_0_EVN.mrc', however, in my process, this file is not generated.
Many appreciation on any suggestions or advice of how it may go wrong.

[navya19j]

I am facing this error too

[Amy1809]

I am facing this error too

Hi Navya, I solved this problem. In the motion correction stage, at the 'Motion' panel, you need to input a 'gain.mrc' file as the blank for Relion to do the blanking.

After I input the gain.mrc file, the motion_corrected_tilt_series.star file has the '_rlnTomoMicrographNameEvn' and 'Odd' files showing up.
I hope this helps.

[navya19j]

Oh wow, where did you get the gain.mrc from?

[Amy1809]

Oh wow, where did you get the gain.mrc from?

In the data I pulled out, there is a file called 'processing', in this file, I get the 'gain.mrc' file.

[navya19j]

Thanks! I tested with that but I still don't see the two columns in my corrected_tilt_series.star. Did you change anything else too?

[Amy1809]

I only changed that bit. Is your file ending in '.mrc' or '.eer'?
Here are my parameters setting:
EER fractionation: 32 (this is according to the tutorial where if you are not using the .EER file, just leave it in 32)
write output in float16?: Yes
Save images for denoising?: Yes
save sum of power spectra?: Yes
sum power spectra every n frames: 4
Bfactor: 150
number of patches X,Y: 1, 1
group frames: 1
binning factor: 1
gain-reference image: gain.mrc (you need to browse to get the correct file in the correct path)
gain rotation: no
gatin flip: no
defect file: I leave this one empty
use RELION's own implementation?: yes

I hope this helps.

[navya19j]

Hi, it's .mrc and I supplied the gain reference image but I still end up with the same error. I am using AreTomo alignment, are you using that as well?

[Amy1809]

Hi, it's .mrc and I supplied the gain reference image but I still end up with the same error. I am using AreTomo alignment, are you using that as well?

Hi, yes I use AreTomo. I suppose you may want to check if there is anything wrong in your previous steps...? I am doing the denoise, so I have also set the "save the images for denoise?" in the motion correction step as "Yes".

[navya19j]

I was able to fix it, Thank you!