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WMB-10X.md

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Clustering analysis of whole adult mouse brain 10X single cell transcriptomes

Clustering analysis of 4.0 million single cell transcriptomes spanning the whole adult mouse brain combining the 10Xv2 and 10Xv3 datasets, resulting in a set of 5196 clusters.

This is one of the steps towards creating a high resolution transcriptomic and spatial atlas of cell types described in Yao et al..

The associated metadata is hosted on AWS S3 bucket as a AWS Public Dataset:

Component Current Version Size
Metadata s3://allen-brain-cell-atlas/metadata/WMB-10X/20231215 2.39 GB

Data is being share under the CC BY NC 4.0 license.

Related resources :

  • Whole mouse brain 10Xv2 single cell transcriptomes (WMB-10Xv2)
  • Whole mouse brain 10Xv3 single cell transcriptomes (WMB-10Xv3)
  • Whole mouse brain 10XMulti single cell transcriptomes (WMB-10XMulti)
  • Whole mouse brain mouse taxonomy of cell types (WMB-taxonomy)

Associated notebooks:

  • Getting started: learn how to use the AbcProjectCache to facilitate data download and usage.
  • 10x scRNA-seq clustering analysis and annotation: learn about the whole mouse brain taxonomy through some example use cases and visualization.
  • 10x scRNA-seq gene expression data
    • Part 1: learn about the 10x dataset through some example use cases and visualization of cells in the thalamus.
    • Part 2a: learn how to iterate through all the data packages, to access data for whole brain example use cases in part 2b.
    • Part 2b: Explore the whole brain data through visualization and analyses of a set of genes of interest.