Nonlinear time-dependent leak current in automated patch-clamp platforms

A study of the nonlinear and time-dependent leak current observed in automated patch-clamp platforms. This repo contains all data and code for reproducing the results in the paper "A nonlinear and time-dependent leak current in the presence of calcium fluoride patch clamp seal enhancer" by Chon Lok Lei, Alan Fabbri, Dominic Whittaker, Michael Clerx, Monique Windley, Adam Hill, Gary Mirams, and Teun de Boer.

Installing

This repository requires Python 3 with Numpy, Scipy, and Matplotlib. An example installation:

python3 -m venv env                # Set up a virtual environment
source env/bin/activate            # Activate the environment
pip install --upgrade pip          # Update pip
pip install -r requirements.txt

Results

• Figure 2: Run figure-2.py.
• Figure 3: Run figure-3.py.
• Figure 4: Run figure-4.py.

Folders

• method: Contains all modules/utility functions.
• data and data-rev: Contains all experimental data and protocols, the individual data files are described in a README in this folder and there is an associated licence.
• fig: All result figures.
• out: Contains all fitting results.

License

All the data provided in this repository within the data and data-rev directory are distributed under a Creative Commons Attribution 4.0 International License. The rest of the code in this repository are under a BSD 3-Clause License.

Acknowledging this work

Please cite this preprint:
Lei CL, Fabbri A, Whittaker DG et al. A nonlinear and time-dependent leak current in the presence of calcium fluoride patch-clamp seal enhancer [version 1; peer review: awaiting peer review]. Wellcome Open Res 2020, 5:152 (https://doi.org/10.12688/wellcomeopenres.15968.1)