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Tublin fluorescence fluctuation data set simulation #1
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Hi, |
Hi, that would be nice to be able to simulate a set of SOFI data, could you suggest me a suitable fluorescence and imaging scheme, I'd be appreciate if you could help me. |
What version of SMIS are you using, and which operating system ? |
Setting up a SOFI experiment is actually very easy: just use EGFP with a 488 laser. EGFP molecules will flicker and bleach progressively. I can send you the .mat SMIS file directly by email if you want ! |
Thanks a lot. My email is: yanru.li@stfc.ac.uk. The version is SMIS 2.2, I think the problem is when I click the «Setup Fluorophore », I can't close this window by clicking Cancle or OK, and some information is different from the manual. |
I am trying to learn to use this software for the purpose of simulating a microtubule dataset of fluorescence fluctuations for reconstruction, but I have just started to get in touch with simulated data and I am not sure if this software can achieve this function? I would be grateful if you could let me know.
Like this one:test_input.zip
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