ETFL is a framework to account for expression, resource allocation, and thermodynamic constraints on stoichiometric models.
You can have a look at our preprint1 on BiorXiv for more information on the formulation and results on an E. coli model.
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Salvy, Pierre, and Vassily Hatzimanikatis. "ETFL: A formulation for flux balance models accounting for expression, thermodynamics, and resource allocation constraints." bioRxiv (2019): 590992.↩