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Review piRNA biogenesis annotations #23
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Notes on biogenesis from One Loop to Rule Them All:The Ping-Pong Cycle and piRNA-Guided Silencing piRNA cluster: clustering of multiple piRNAs at distinct genomic loci, resulting in sites with high piRNA coverage. Cytologically, mouse clusters reside in euchromatic domains, whereas clusters in flies are embedded in heterochromatin and predominantly found at pericentromeric and subtelomeric regions. Primary piRNA biogenesis can be broken down into several steps:
Mature piRNA-PIWI complexes have different fates depending on the protein involved The localization of these Argonaute proteins influences the mode of action by which they carry out silencing
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NTR? : TRANSPOSON SILENCING Asterix/Gtsf1 links tRNAs and piRNA silencing of retrotransposons |
I think that would make sense. piRNAs act via transposon silencing and if tRNAs also act on transposons, then it would make sense to have the new term as a common parent, I think. |
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Additional task to review regulation of transposition https://docs.google.com/spreadsheets/d/1F_70d1hpogIfEoSiC-8FRvVy4HXCTwduJGBVhg8TOuY/edit#gid=0 |
Sheet with papers for curation for biogenesis |
Fly piRNA biogenesis: tap dancing with Tej looks at the difference between where primary and secondary processing occurs: In short, primary and secondary processing are separate processes. |
Note: came across these terms: |
Useful description of piRNA - piwi complexes and their specificity in this article |
Draft definition for primary piRNA processing The process involved in converting precursor piRNAs (long single-stranded capped and polyadenylated RNAs) into non-overlapping, contiguous primary piRNAs (∼24-30-nt piRNAs with a preference for a 5' uridine (U) via the endonucleolytic activity of cytosolic PIWI. This may include pre-piRNA maturation of 3′ ends by trimming and 2′-O-methylation. Refs: PMID:34469728 Might change the def to remove mention of U as in mouse this preference might not be so strong - need to check (https://www.science.org/doi/10.1126/science.aaa1039) .....Update, U bias ok for mice too https://www.nature.com/articles/cr2012120 |
From PMID: 19270082 "The third class of small RNAs appears to be specific to animals and interacts with an animal-specific clade of Argonaute proteins, the Piwi family (Aravin et al. 2007a). These Piwi-interacting RNAs (piRNAs) have been found in Caenorhabditis elegans (Batista et al. 2008; Das et al. 2008), Drosophila (Saito et al. 2006; Brennecke et al. 2007), zebra fish (Houwing et al. 2007, 2008), and mam- mals (Aravin et al. 2006, 2007b; Girard et al. 2006; Grivna et al. 2006; Lau et al. 2006; Watanabe et al. 2006), where their expression is most prominent in male and female germ cells. In most organisms, piRNAs have clear roles in guarding germ cell genomes from the activity of mobile genetic elements ...." Taxon restriction for all piRNA terms should be: |
draft for Secondary piRNA processing Note: official name of MIWI2 is Piwil4 and MILI is Piwil2 (http://www.informatics.jax.org/marker/MGI:1930036) |
May have to have a Tertiary piRNA processing term for phased piRNA production but need more papers to get definition correct. |
https://www.tandfonline.com/doi/full/10.4161/worm.28234 |
Well, that makes it easier!:) |
Some Rules/giudes for annotation (add to this list)
Note for secondary processing
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Hi @pgaudet I think from looking over the papers Giulia and I think that it would be safer to have a parent term (piRNA processing) for primary piRNA processing or secondary terms. The reasoning behind this is that although they can be and mainly are separate processes, there are examples where they may feed into each other or that authors are just not clear. And, if we were to make a term for tertiary processing (and I am not sure about whether we would want that), it would have to be under a generic parent as it can't take place without primary or secondary processing first. |
https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6601507&blobtype=pdf |
making sure all components are covered. |
see GO ticket go-ontology/issues/23135
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