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Hoohm committed Mar 17, 2017
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Expand Up @@ -13,8 +13,6 @@ This pipeline is based on [snakemake](https://snakemake.readthedocs.io/en/stable

Installation
--------
This small pipeline allows you to run the basic steps to align and extract expression from a drop-seq experiment.

Before using it you will need to install some softwares/packages:

1. [R](https://cran.r-project.org/)
Expand Down Expand Up @@ -100,7 +98,7 @@ Note: The name of the species is relevant in the mixed experiment, it has to mat

Once everything is in place, you can run the pipeline using the following command:

`python3 dropSeqPip -f /path/to/your/samples/ -c /path/to/local/config/file.yaml -m mode`
`dropSeqPip -f /path/to/your/samples/ -c /path/to/local/config/file.yaml -m mode`

You can choose from four different modes to run:

Expand All @@ -111,14 +109,14 @@ You can choose from four different modes to run:

If you don't need to change values in the config files for the different steps, you can also simply run multiple modes at a time. ie:

`python3 dropSeqPip -f /path/to/your/samples/ -c /path/to/local/config/file.yaml -m pre-process knee-plot extract-expression`
`dropSeqPip -f /path/to/your/samples/ -c /path/to/local/config/file.yaml -m pre-process knee-plot extract-expression`

This is the folder structure you get in the end:
```
/path/to/your/samples/
| -- plots
| -- summary
| -- logs
| -- plots/
| -- summary/
| -- logs/
| config.yaml
```

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