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EM-stitching

Some scripts for single-beam ssSEM images stitching based on Rhoana's rh-aligner.

System

Ubuntu 18.04

Enviroment preparation

  • Download the relevant packages.
    git clone git@github.com:horacekem/singlebeam-EM-stitching(this repo)
    git clone git@github.com:Rhoana/tinyr
    git clone git@github.com:Rhoana/rh_renderer
  • Create conda environment.
    conda create single_stitch -n python=3.7
    conda activate single_stitch
  • Switch to this repo's folder
    pip install -r requirements.txt
    conda install opencv=3.4.2
  • Switch to tinyr's folder
    python setup.py install
  • Switch to rh_renderer's folder
    export PKG_CONFIG_PATH=/home/YOUR_USER_NAME/anaconda3/envs/singe_stitch/lib/pkgconfig/
    python setup.py install

How to

  • Switch to this repo's folder/source
  • Modify the arguments in arguments.txt
  • Run stitching_driver.py