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Small variations in timing of spikes produce several clusters #510

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guilhemibos opened this issue Jul 6, 2022 · 2 comments · Fixed by #595
Closed

Small variations in timing of spikes produce several clusters #510

guilhemibos opened this issue Jul 6, 2022 · 2 comments · Fixed by #595

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@guilhemibos
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Hi

I'm new to Kilosort. I'm using KS3, recording from 32 channels linear stereotrodes (50um between contacts), with open pehys. I have a hard time calibrating KS in order to have decent results which do not need very long manual curation.

Often, I observe some sorting like this:
A large spike is separated arbitrarily into 2 or more units because their is a small temporal drift in the waveform, messing up with correlagrams of neurons. Is there a reason why this happens and how can I get rid of this issue?
Thanks
Screenshot from 2022-07-06 15-59-17

Screenshot from 2022-07-06 16-00-01

Screenshot from 2022-07-06 15-50-01

@ananmoran
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Hi,
I just want to add that I encounter exactly the same phenomenon sorting Neuropixels data recorded with SpikeGLX. I hope someone can help to resolve this issue.
Thanks
Anan

@shirquinn
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@guilhemibos Did you manage to solve the problem? can you share what you did? I'm having a similar issue

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3 participants