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Stability test - Inlet port 3 - Outlet port 2 - 1 step - 96 cycles - 8 mins-cycle - Qdraw 100 pulses-sec Qpump 2 pulses-sec - Period 16 cycles.txt
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Stability test - Inlet port 3 - Outlet port 2 - 1 step - 96 cycles - 8 mins-cycle - Qdraw 100 pulses-sec Qpump 2 pulses-sec - Period 16 cycles.txt
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#-------------------------------------------------------
# Stability script
#
# Version: 2.1
# Author: Dimitrios Simatos
# Last updated: 2022-11-16
# Changes: Updated the duration of script with the measured duration of script.
#-------------------------------------------------------
# Parameters
#
# Input port sequence: 3
# Outlet port sequence: 2
# Number of steps (step: an integer number of cycles during which a specific liquid is injected): 1
# Number of cycles: 96
# Cycles per step: 96
# Cycle time: 8 minutes
# Period (number of cycles between pumping cycles - even of the same liquid): 16
# Liquid injections per step (cycles per step/period): 6
# Duration of script: Theoretical: 12.8 hours, Measured: 15 hours and 25 minutes
#
# Syringe size: 1 ml
# Step resolution: N = 0.01 mm
# Drawing flow (Qdraw): V = 100 pulses/second (2000 ul/min)
# Pumping flow (Qpump): V = 2 pulses/second (40 ul/min)
# - Time to empty syringe: Syringe size/Qpump = 25 minutes
#-------------------------------------------------------
# Description
#
# This script is designed to pump a liquid from a single port into the flow cell.
# The script allows the baseline 2 hours to stabilize and then flushes the flow cell with liquid.
# The script was made to see if the baseline stability depends on liquid being flushed inside the flow cell (and hence if the liquid's conductivity changes with time if it remains still inside the flow cell).
#-------------------------------------------------------
# Important points
#
# Never leave any liquid in the pump to dry after the experiments. If they dry, the residue may block the valve.
# Always run the cleaning script after the experiments.
# Use clockwise rotation when going up in valve port numbers and vice versa.
# Do not tighten the reservoir caps. It may lead to overpressure of air.
#-------------------------------------------------------
# Things to do before running the script
#
# Set time units to minutes.
# Set filenames.
# Replace the tubing, if necessary.
#-------------------------------------------------------
# Port connections
#
# Port 1: Waste
# Port 2: Device
# Port 3: Ultrapure water
# Port 4: Nothing
# Port 5: Nothing
# Port 6: Nothing
#-------------------------------------------------------
# Pumping cycles and execution time
#
# There is only 1 step, with 96 cycles.
# Every 2 hours (16 cycles) new liquid is flushed inside the flow cell.
# Every cycle is 8 minutes long. The total number of cycles is 96.
# All cycles pump DI water from port 3.
#
# Execution time for a single cycle: 8 minutes
# Total (theoretical) execution time: 1 (initialization) + 96 x 8 = 769 minutes = 12.8 hours
# Total (measured) execution time: 15 hours and 25 minutes
#-------------------------------------------------------
# Volume of liquid needed
#
# Volume of liquid needed per step: 1 ml
# Total volume of liquid needed per script run (volume/cycle * cycles in which the liquid is injected):
# 1 ml x 6 = 6 ml ultrapure water
#-------------------------------------------------------
# SPA settings
itime l 0.04
gaterangemode l
drainrangemode l
#-------------------------------------------------------
# Pump initialization
pump /1T #hard stop pump
pump /1ZR #initialize pump
wait-min 1 #wait 1 minute
#-------------------------------------------------------
# Cycle 1: Pumping the liquid of port 3 (8 minutes)
filename step_1_up-H2O_initial_
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1N0R #set step resolution: 0.01 mm
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be at 0)
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait 1000 #wait 1 second
pump /1V100R #choose speed: 100 steps/s (2000 ul/min)
pump /1Q #query pump
wait 1000 #wait 1 second
pump /1I3R #go to port 3, clockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1A3000R #go to absolute position 3000 (= draw 1 ml)
wait-min 1 #wait 1 minute
pump /1V2R #set speed to 2 steps/s (40 ul/min)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should have finished moving)
#pump /1?6 #report valve position
pump /1O2R #switch to port 2, counterclockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
pump /1A100R #go to absolute position 100 (do not fully empty syringe)
#pump /1A0R #go to absolute position 0 (= empty syringe)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be moving to 0)
#pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be moving to 0)
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
#-------------------------------------------------------
# Cycle 2: Measuring for 8 minutes
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
#-------------------------------------------------------
# Cycle 3: Measuring for 8 minutes
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
#-------------------------------------------------------
# Cycle 4: Measuring for 8 minutes
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
#-------------------------------------------------------
# Cycle 5: Measuring for 8 minutes
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
#-------------------------------------------------------
# Cycle 6: Measuring for 8 minutes
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
#-------------------------------------------------------
# Cycle 7: Measuring for 8 minutes
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
#-------------------------------------------------------
# Cycle 8: Measuring for 8 minutes
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
#-------------------------------------------------------
# Cycle 9: Measuring for 8 minutes
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
#-------------------------------------------------------
# Cycle 10: Measuring for 8 minutes
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
#-------------------------------------------------------
# Cycle 11: Measuring for 8 minutes
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
#-------------------------------------------------------
# Cycle 12: Measuring for 8 minutes
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
#-------------------------------------------------------
# Cycle 13: Measuring for 8 minutes
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
#-------------------------------------------------------
# Cycle 14: Measuring for 8 minutes
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
#-------------------------------------------------------
# Cycle 15: Measuring for 8 minutes
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
#-------------------------------------------------------
# Cycle 16: Measuring for 8 minutes
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
#-------------------------------------------------------
# Cycle 17: Pumping the liquid of port 3 (8 minutes)
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1N0R #set step resolution: 0.01 mm
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be at 0)
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait 1000 #wait 1 second
pump /1V100R #choose speed: 100 steps/s (2000 ul/min)
pump /1Q #query pump
wait 1000 #wait 1 second
pump /1I3R #go to port 3, clockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
wait 1000 #wait 1 second
pump /1A3000R #go to absolute position 3000 (= draw 1 ml)
wait-min 1 #wait 1 minute
pump /1V2R #set speed to 2 steps/s (40 ul/min)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should have finished moving)
#pump /1?6 #report valve position
pump /1O2R #switch to port 2, counterclockwise rotation
pump /1Q #query pump
pump /1?6 #report valve position
pump /1A100R #go to absolute position 100 (do not fully empty syringe)
#pump /1A0R #go to absolute position 0 (= empty syringe)
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be moving to 0)
#pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position (the plunger should be moving to 0)
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
#-------------------------------------------------------
# Cycle 18: Measuring for 8 minutes
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
#-------------------------------------------------------
# Cycle 19: Measuring for 8 minutes
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
#-------------------------------------------------------
# Cycle 20: Measuring for 8 minutes
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
#-------------------------------------------------------
# Cycle 21: Measuring for 8 minutes
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
#-------------------------------------------------------
# Cycle 22: Measuring for 8 minutes
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position
pump /1?6 #report valve position
transfer d 0 -1 -0.1 -1 -1 0
wait-min 1 #wait 1 minute
pump /1Q #query pump
pump /1? #report absolute plunger position