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trim to the right of the first restriction site, and discard reads that after trimming are below 50 bp to ensure decent confidence when aligning them.
trimming_settings="ktrim=r k=4 mink=2 hdist=0 qin=33 minlen=50 tossbrokenreads" #-t
reads on genes with the trimmed data (with the restriction sites)=2640422
reads on genes with the NON trimmed data (with the restriction sites)=5041605
insertions on genes with the trimmed data (with the restriction sites)=215235
insertions on genes with the NON trimmed data (with the restriction sites)=342014
the number of reads in the ADE2 for the trimmed one is around 7% of the total amount of reads with 187507 and for the non trimmed one it represents 20% of the data with 1003795.
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