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Reanalyze all our datasets including the trimming of the restriction sites as a preprocessing step #5

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leilaicruz opened this issue Feb 24, 2022 · 1 comment

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@leilaicruz leilaicruz created this issue from a note in Data analysis tasks (To do) Feb 24, 2022
@leilaicruz leilaicruz moved this from To do to In progress in Data analysis tasks Feb 24, 2022
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leilaicruz commented Feb 24, 2022

  • dbem3_a
    Settings for the trimming :

trim to the right of the first restriction site, and discard reads that after trimming are below 50 bp to ensure decent confidence when aligning them.
trimming_settings="ktrim=r k=4 mink=2 hdist=0 qin=33 minlen=50 tossbrokenreads" #-t

  • reads on genes with the trimmed data (with the restriction sites)=2640422
  • reads on genes with the NON trimmed data (with the restriction sites)=5041605
  • insertions on genes with the trimmed data (with the restriction sites)=215235
  • insertions on genes with the NON trimmed data (with the restriction sites)=342014
  • the number of reads in the ADE2 for the trimmed one is around 7% of the total amount of reads with 187507 and for the non trimmed one it represents 20% of the data with 1003795.
    igv_snapshot

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