Signaling Profiler is a R based pipeline that integrates transcriptomics, proteomics and phosphoproteomics data among a case and a control to build a signaling network, connecting:
- cellular receptor(s);
- kinases and phosphatases;
- targets of phosphorylation;
- transcription factors;
This network provides mechanistics hypoteses about the remodeling of the signal due to your case.
To install Signaling Profiler execute the following command in R.
R >= 4.1 is required
devtools::install_github('https://github.com/SaccoPerfettoLab/SignalingProfiler/')SignalingProfiler is currently under active development, as such we suggest to delete and re-install the package regularly to exploit the new features.
Signaling Profiler is dependent on Saez lab algorithm CARNIVAL for performing the signalling pathway optimization. You can find all the instruction for installation in Saez lab repository CARNIVAL.
You can find the tutorial of Signaling Profiler here.
A new tutorial for human analysis is available here
If you use SignalingProfiler for your research please cite the original publication.
Massacci, G., Venafra, V., Latini, S. et al. A key role of the WEE1-CDK1 axis in mediating TKI-therapy resistance in FLT3-ITD positive acute myeloid leukemia patients. Leukemia (2022). https://doi.org/10.1038/s41375-022-01785-w
