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Single Cell Imaging Software
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Wrobel J, Harris C, Vandekar S. Statistical Analysis of Multiplex Immunofluorescence and Immunohistochemistry Imaging Data. Methods in Molecular Biology 2023. 2629: 141--168. DOI
Software Information Functionality
Name Purpose Language Store Viz Norm Seg Ptype Spatial
VectraPolarisData Example mIF datasets R (Bioc)
histoCAT Computational histology topography cytometry analysis toolbox MATLAB, GUI
Squidpy Tool for the analysis and visualization of spatial molecular data Python
Giotto Tools to process, analyze and visualize single-cell spatial expression data R, GUI, Python depends
imcRTools Analysis of imaging mass cytometry and other highly multiplexed data R (Bioc)
SVCA Computational framework for the analysis of spatial molecular data Python, R depends
SPIAT Data processing, quality control, visualization, data analysis tools R
spatialTIME Spatial analysis of mIF data R
mxnorm Normalization techniques for mIF data R, Python depends
Seurat Toolkit for QC, analysis, and exploration of single-cell omics data R
QuPath Powerful, flexible, extensible platform for bioimage analysis GUI, Java
HALO Platform for quantitative tissue analysis in digital pathology Closed source, GUI
inForm Visualizing, segmenting, and phenotyping images from Vectra instruments Closed source, GUI
Cytomapper Visualization of cell-level info obtained by multiplexed imaging cytometry R (Bioc)
CyLinter Quality control software for multiplex microscopy Python
CellProfiler Interactive data analysis for highthroughput, image-based experiments Python