Animation of Blender's Bezier Curves and Grease Pencil on top of a Background Image:
The initial Blender implementation was written as a TKinter external application to eventually duplicate the 2016 Workshop demostration used by CellBlender:
That model was simulated with a series of fabricated "slices":
The slices were then (quickly) traced in Reconstruct to produce a 3D model:
The model was exported from Reconstruct as VRML 2.0 and imported into Blender:
The "Image Tracer" Blender addon displays one slice for tracing to eventually build a full 3D model in Blender: