PCR duplicates removal

[seven1112233]

Hi,
If we investigate splicing kinetics for the nanopore cDNA sequencing data (FLEP-seq), should we perform PCR removal?
I am worried that the PCR amplification would affect the splicing analysis.
You know, during PCR amplification, certain DNA fragments may be preferentially amplified over others, leading to an overrepresentation of some sequences in the final sequencing data. This bias can result in an inaccurate estimation of the true abundance of different sequences, including variants.