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Basecalling

  • Install Nvidia Drivers and Guppy on a g4dn.xlarge AWS instance with Ubuntu 18.08
    • bash install_guppy.sh
  • Basecall using simple script. Note: this will only work if you untar our fast5 files with their corresponding directory structure inside the fast5 directory. See end_to_end
    • bash /home/ubuntu/yeast_rrna_modification_detection/basecalling/run_guppy.sh /home/ubuntu/fast5 /home/ubuntu/fastq