From Enochs et al. 2023
1. Field collections
In June 2021, four genotypes of A. cervicornis were outplanted to the urban Coral City Camera site following collection from the in-situ coral nursery off Key Biscayne, FL. In late October/early November 2021, ~1 cm tissue samples were collected from each surviving outplant and an offshore in-situ nursery conspecific and immediately preserved in Zymo DNA/RNA Shield.
Figure 2. Images from Dalton Hesley of initial outplants in June 2021 with colored zipties for genotype tracking, and different outplants after six months in the field (Nov 2021).
2. Extractions and Sequencing
Total RNA was extracted using the Zymo MagBead DNA/RNA extraction kit, then prepared as QuantSeq 3'mRNA-Seq cDNA libraries and sequenced on a NOVAseq S2 flow cell at the University of Miami Genomics Center.
3. Bioinformatics
See this README for in-depth scripts of pipeline.