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<PubmedArticle> <MedlineCitation Status="MEDLINE" Owner="NLM"> <PMID Version="1">31358213</PMID> <DateCompleted> <Year>2019</Year> <Month>10</Month> <Day>07</Day> </DateCompleted> <DateRevised> <Year>2019</Year> <Month>10</Month> <Day>07</Day> </DateRevised> <Article PubModel="Print-Electronic"> <Journal> <ISSN IssnType="Electronic">1873-4324</ISSN> <JournalIssue CitedMedium="Internet"> <Volume>1078</Volume> <PubDate> <Year>2019</Year> <Month>Oct</Month> <Day>31</Day> </PubDate> </JournalIssue> <Title>Analytica chimica acta</Title> <ISOAbbreviation>Anal Chim Acta</ISOAbbreviation> </Journal> <ArticleTitle>Gold nanoparticle triggered siloxane formation for polymerization based amplification in enzyme free visual immunoassay.</ArticleTitle> <Pagination> <MedlinePgn>151-160</MedlinePgn> </Pagination> <ELocationID EIdType="pii" ValidYN="Y">S0003-2670(19)30739-1</ELocationID> <ELocationID EIdType="doi" ValidYN="Y">10.1016/j.aca.2019.06.017</ELocationID> <Abstract> <AbstractText>Herein, we report a new signal amplification scheme for quantitative biochemical analysis based on gold nanoparticle (GNPs) catalyzed polymerization of transparent silane solution to milky white and turbid siloxane. Using immunoassay as a proof of concept, GNP labeled immunoprobe was used to bind captured antigen and catalyse the polymerization reaction allowing sensitive biochemical investigation. The polymerization reaction was optimized for standard 96 well polystyrene microtiter plates and we discovered that sodium lactate acts as an enhancer in the polymerization reaction as it reduces detection time to merely 30 min. The sensing strategy was applied to detection and quantification of Salmonella Typhimurium in water and egg samples and the platform showed excellent visibly quantifiable analytical response up to 100 cells mL<sup>-1</sup>. Furthermore, clinical utility and potential of the method was validated by detecting Vi capsular polysaccharide (Vi antigen) responsible for typhoidal Salmonellosis in human serum in sandwich format with a detection limit of 1 ng mL<sup>-1</sup>. The method serves as the first report towards nanoparticle triggered polymerization for development of rapid and low cost quantitative biochemical assay.</AbstractText> <CopyrightInformation>Copyright © 2019 Elsevier B.V. All rights reserved.</CopyrightInformation> </Abstract> <AuthorList CompleteYN="Y"> <Author ValidYN="Y"> <LastName>Singh</LastName> <ForeName>Pargat</ForeName> <Initials>P</Initials> <AffiliationInfo> <Affiliation>UIET-Panjab University, Sector-25, Chandigarh, India; CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh, India.</Affiliation> </AffiliationInfo> </Author> <Author ValidYN="Y"> <LastName>Bharti</LastName> <AffiliationInfo> <Affiliation>CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh, India.</Affiliation> </AffiliationInfo> </Author> <Author ValidYN="Y"> <LastName>Kumar</LastName> <ForeName>Rajesh</ForeName> <Initials>R</Initials> <AffiliationInfo> <Affiliation>UIET-Panjab University, Sector-25, Chandigarh, India.</Affiliation> </AffiliationInfo> </Author> <Author ValidYN="Y"> <LastName>Bhalla</LastName> <ForeName>Vijayender</ForeName> <Initials>V</Initials> <AffiliationInfo> <Affiliation>CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh, India. Electronic address: vkbhalla@imtech.res.in.</Affiliation> </AffiliationInfo> </Author> </AuthorList> <Language>eng</Language> <PublicationTypeList> <PublicationType UI="D016428">Journal Article</PublicationType> </PublicationTypeList> <ArticleDate DateType="Electronic"> <Year>2019</Year> <Month>06</Month> <Day>10</Day> </ArticleDate> </Article> <MedlineJournalInfo> <Country>Netherlands</Country> <MedlineTA>Anal Chim Acta</MedlineTA> <NlmUniqueID>0370534</NlmUniqueID> <ISSNLinking>0003-2670</ISSNLinking> </MedlineJournalInfo> <ChemicalList> <Chemical> <RegistryNumber>0</RegistryNumber> <NameOfSubstance UI="D000906">Antibodies</NameOfSubstance> </Chemical> <Chemical> <RegistryNumber>0</RegistryNumber> <NameOfSubstance UI="D060766">Drinking Water</NameOfSubstance> </Chemical> <Chemical> <RegistryNumber>0</RegistryNumber> <NameOfSubstance UI="D011135">Polysaccharides, Bacterial</NameOfSubstance> </Chemical> <Chemical> <RegistryNumber>0</RegistryNumber> <NameOfSubstance UI="D012821">Silanes</NameOfSubstance> </Chemical> <Chemical> <RegistryNumber>0</RegistryNumber> <NameOfSubstance UI="D012833">Siloxanes</NameOfSubstance> </Chemical> <Chemical> <RegistryNumber>0</RegistryNumber> <NameOfSubstance UI="C054154">capsular polysaccharide, Salmonella</NameOfSubstance> </Chemical> <Chemical> <RegistryNumber>7440-57-5</RegistryNumber> <NameOfSubstance UI="D006046">Gold</NameOfSubstance> </Chemical> </ChemicalList> <CitationSubset>IM</CitationSubset> <MeshHeadingList> <MeshHeading> <DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName> </MeshHeading> <MeshHeading> <DescriptorName UI="D000906" MajorTopicYN="N">Antibodies</DescriptorName> <QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName> </MeshHeading> <MeshHeading> <DescriptorName UI="D002645" MajorTopicYN="N">Chickens</DescriptorName> </MeshHeading> <MeshHeading> <DescriptorName UI="D060766" MajorTopicYN="N">Drinking Water</DescriptorName> <QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName> </MeshHeading> <MeshHeading> <DescriptorName UI="D004531" MajorTopicYN="N">Eggs</DescriptorName> <QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName> </MeshHeading> <MeshHeading> <DescriptorName UI="D005506" MajorTopicYN="N">Food Contamination</DescriptorName> <QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName> </MeshHeading> <MeshHeading> <DescriptorName UI="D006046" MajorTopicYN="N">Gold</DescriptorName> <QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName> </MeshHeading> <MeshHeading> <DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName> </MeshHeading> <MeshHeading> <DescriptorName UI="D007118" MajorTopicYN="N">Immunoassay</DescriptorName> <QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName> </MeshHeading> <MeshHeading> <DescriptorName UI="D057230" MajorTopicYN="N">Limit of Detection</DescriptorName> </MeshHeading> <MeshHeading> <DescriptorName UI="D053768" MajorTopicYN="N">Metal Nanoparticles</DescriptorName> <QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName> </MeshHeading> <MeshHeading> <DescriptorName UI="D010316" MajorTopicYN="N">Particle Size</DescriptorName> </MeshHeading> <MeshHeading> <DescriptorName UI="D058105" MajorTopicYN="N">Polymerization</DescriptorName> </MeshHeading> <MeshHeading> <DescriptorName UI="D011135" MajorTopicYN="N">Polysaccharides, Bacterial</DescriptorName> <QualifierName UI="Q000097" MajorTopicYN="Y">blood</QualifierName> <QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName> </MeshHeading> <MeshHeading> <DescriptorName UI="D000075082" MajorTopicYN="N">Proof of Concept Study</DescriptorName> </MeshHeading> <MeshHeading> <DescriptorName UI="D012486" MajorTopicYN="N">Salmonella typhimurium</DescriptorName> <QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName> <QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName> </MeshHeading> <MeshHeading> <DescriptorName UI="D012821" MajorTopicYN="N">Silanes</DescriptorName> <QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName> </MeshHeading> <MeshHeading> <DescriptorName UI="D012833" MajorTopicYN="N">Siloxanes</DescriptorName> <QualifierName UI="Q000138" MajorTopicYN="Y">chemical synthesis</QualifierName> </MeshHeading> <MeshHeading> <DescriptorName UI="D013696" MajorTopicYN="N">Temperature</DescriptorName> </MeshHeading> </MeshHeadingList> <KeywordList Owner="NOTNLM"> <Keyword MajorTopicYN="N">Gold nanoparticles</Keyword> <Keyword MajorTopicYN="N">Immunoassay</Keyword> <Keyword MajorTopicYN="N">Polymerization</Keyword> <Keyword MajorTopicYN="N">Salmonella typhimurium</Keyword> <Keyword MajorTopicYN="N">Vi capsular polysaccharide</Keyword> </KeywordList> </MedlineCitation> <PubmedData> <History> <PubMedPubDate PubStatus="received"> <Year>2019</Year> <Month>03</Month> <Day>01</Day> </PubMedPubDate> <PubMedPubDate PubStatus="revised"> <Year>2019</Year> <Month>06</Month> <Day>06</Day> </PubMedPubDate> <PubMedPubDate PubStatus="accepted"> <Year>2019</Year> <Month>06</Month> <Day>07</Day> </PubMedPubDate> <PubMedPubDate PubStatus="entrez"> <Year>2019</Year> <Month>7</Month> <Day>31</Day> <Hour>6</Hour> <Minute>0</Minute> </PubMedPubDate> <PubMedPubDate PubStatus="pubmed"> <Year>2019</Year> <Month>7</Month> <Day>31</Day> <Hour>6</Hour> <Minute>0</Minute> </PubMedPubDate> <PubMedPubDate PubStatus="medline"> <Year>2019</Year> <Month>10</Month> <Day>8</Day> <Hour>6</Hour> <Minute>0</Minute> </PubMedPubDate> </History> <PublicationStatus>ppublish</PublicationStatus> <ArticleIdList> <ArticleId IdType="pubmed">31358213</ArticleId> <ArticleId IdType="pii">S0003-2670(19)30739-1</ArticleId> <ArticleId IdType="doi">10.1016/j.aca.2019.06.017</ArticleId> </ArticleIdList> </PubmedData> </PubmedArticle>