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soap didn't read fastq files in Pregraph and Contig #84

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sqwwww opened this issue Jun 29, 2022 · 2 comments
Closed

soap didn't read fastq files in Pregraph and Contig #84

sqwwww opened this issue Jun 29, 2022 · 2 comments

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@sqwwww
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sqwwww commented Jun 29, 2022

hello, the soap denovo didn't read fastq files in Pregraph and Contig, but can read fastq files in Map step, could you give some suggestion about the situation?
the ouput is the following log.txt. Thanks for your patience.
log.txt

@sqwwww
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sqwwww commented Jul 1, 2022

I tested the prlHashReads.c, and found the "openNextFile" function returns 0, and thus it didn't read any fastq files. I'm not sure if it's about some problem in my fastq files, but the fastq files can pass fastqc normally.

@sqwwww
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sqwwww commented Jul 1, 2022

I got it, it's related to "asm_flags" in the config file, I originally set it as 2 because I only want to assemble the scaffold but not contig, and then I met the problem I mentioned above. When I set it as 3, all the thing goes normally. so I guess the scaffold should be run after contig, it's a successive process but not a selection.

@sqwwww sqwwww closed this as completed Jul 1, 2022
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