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Smoove Lumpy error in structural variation pipeline #3633
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I just did a little digging in my system to find how many samtools executables are there in the bcbio directory using this command:
When I executed each one of them, these samtools executables gave various shared libraries errors: I was thinking if the lumpy error is due to samtools shared library errors as I saw quite some samtools errors in the error log and the pipeline began to fail just after that. |
So, the last command which ran was
No wonder, I got samtools error because the samtools present in When I just ran the above code, but changed the samtools path to the one which was working, the command ran fine, until it had another error for bcftools: There are many samtools/bcftools instances installed which different programs access differently. Is there a workaround to tackle such an issue. How can I specify that the pipeline accesses only the software which is working? Actually, the question should be- why are not all the software working and getting this shared library errors? Any workarounds would be greatly appreciated. Is this bcbio v1.2.9 specific? Regards, |
After some re-installations/updates, I was able to make both samtools/bcftools run in both default conda and python2 environment. I followed this solution and updated samtools to v1.9: merenlab/anvio#1479 I updated bcftools too and tested to check if both tools ran succesfully in both environments or not. |
Version info
bcbio_nextgen.py --version
): v1.2.9lsb_release -ds
): Red Hat Enterprise Linux release 8.5 (Ootpa)To Reproduce
Exact bcbio command you have used:
Your yaml configuration file:
Log files (could be found in work/log)
Please attach (10MB max):
bcbio-nextgen-commands.log
, andbcbio-nextgen-debug.log
.bcbio-nextgen-commands.log
bcbio-nextgen-debug.log
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